5-Ethynyl-2′-deoxyuridine (EdU) 是一种胸腺嘧啶核苷类似物,在细胞增殖时能够插入正在复制的 DNA 分子中, EdU 与染料的共轭反应可以进行高效快速的细胞增殖检测分析。5-Ethynyl-2′-deoxyuridine 是一种 PROTAC linker,属于 alkyl chain 类。可用于合成 PROTAC 分子。
5-Ethynyl-2′-deoxyuridine Chemical Structure
CAS No. : 61135-33-9
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生物活性
5-Ethynyl-2′-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation[1]. 5-Ethynyl-2′-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs[2].
IC50 & Target
Alkyl-Chain
体外研究 (In Vitro)
5-Ethynyl-2′-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system[1]. PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
The number of 5-Ethynyl-2′-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two month old female mice[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Two month old female mice[1]
Dosage:
10, 20, 50, 100 or 200 mg/kg
Administration:
Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.
Result:
EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.
分子量
252.22
Formula
C11H12N2O5
CAS 号
61135-33-9
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Chenbo Zeng, et al. Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.
[2]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562