Scoulerine(Synonyms: (-)-Scoulerine; Discretamine)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Scoulerine (Synonyms: (-)-Scoulerine; Discretamine) 纯度: 99.27%

Scoulerine ((-)-Scoulerine) 是一种有效的抗有丝分裂 (antimitotic) 化合物。Scoulerine 也是 BACE1 (淀粉样前体蛋白裂解酶 1) 的抑制剂。Scoulerine 抑制细胞增殖，阻止细胞周期，并诱导癌细胞凋亡。

Scoulerine(Synonyms: (-)-Scoulerine; Discretamine)

Scoulerine Chemical Structure

CAS No. : 6451-73-6

规格 价格 是否有货 数量
1 mg ￥2600 In-stock
5 mg   询价  
10 mg   询价  

* Please select Quantity before adding items.

生物活性

Scoulerine ((-)-Scoulerine), an isoquinoline alkaloid, is a potent antimitotic compound. Scoulerine is also an inhibitor of BACE1 (ß-site amyloid precursor protein cleaving enzyme 1). Scoulerine inhibits proliferation, arrests cell cycle, and induces apoptosis in cancer cells[1].

体外研究
(In Vitro)

Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC50s ranging from 2.7 µM to 6.5 µM[1].
Scoulerine (2.5-20 µM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells[1].
Scoulerine (2.5-20 µM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis[1].
Scoulerine induces G2 or M cell cycle arrest[1].
Scoulerine (2.5-5 µM; 24 hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells[1].
Scoulerine (2.5-5 µM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner[1].
Scoulerine (5-10 µM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells[1]

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Jurkat and MOLT-4 cells
Concentration: 2.5, 5, 10, 15 and 20 µM
Incubation Time: 24 hours
Result: Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner.

Apoptosis Analysis[1]

Cell Line: MOLT-4 and Jurkat cells
Concentration: 2.5, 5, 10, 15 and 20 µM
Incubation Time: 24 hours
Result: Induced MOLT-4 and Jurkat cells apoptosis.

Cell Cycle Analysis[1]

Cell Line: Jurkat and MOLT-4 leukemic cells
Concentration: 2.5-20 µM
Incubation Time: 16 hours
Result: Induced cell cycle arrest at the G2/M transition.

Western Blot Analysis[1]

Cell Line: MOLT-4 cells
Concentration: 2.5, 5 µM
Incubation Time: 24 hours
Result: Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells.

分子量

327.37

Formula

C19H21NO4
CAS 号

6451-73-6
中文名称

金黄紫堇碱
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
参考文献

  • [1]. Habartova K, et al. Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci Rep. 2018;8(1):4829. Published 2018 Mar 19.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务