Ethyl gallate is a nonflavonoid phenolic compound and also a scavenger of hydrogen peroxide.

Ethyl gallate is a nonflavonoid phenolic compound and also a scavenger of hydrogen peroxide. After treatment for 24 h or 48 h with Ethyl gallate, HL-60 cells show changes in morphology, including shrinkage of the cell membrane and the development of apoptotic bodies. Consistent with these effects, the viability of Ethyl gallate-treated cells decreases in a time- and dose-dependent manner, demonstrating that Ethyl gallate has a cytotoxic effect on HL-60 cells. Ethyl gallate treatment increases the proportion of cells in subG1 phase in a concentration- and time-dependent manner. Treatment of cells for 24 h or 48 h with 50 μM or 75 μM Ethyl gallate increases the percentage of cells in the subG1 phase from a baseline of 2.9% to 26.5% or 52.6%, respectively. It is found that Ethyl gallate treatment of HL-60 cells decreases the expression of Bcl-2 at 75 μM Ethyl gallate, and increases Bax and truncated Bid (tBid) expression at 24 h^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

No significant difference in the serum total protein, albumin, globulin and glucose is found between the rats fed with A. nilotica (L.) leaf extract on ethyl gallate equivalent basis and those fed with Ethyl gallate alone. Significant differences in total bilirubin level, however, exist between the rats that receive A. nilotica (L.) leaf extract, 500 mg/kg body weight (ethyl gallate equivalent of 10 mg/kg, 0.34±0.01 mg/dL) and those receiving 10 mg/kg body weight of Ethyl gallate (0.26±0.01 mg/dL). Significant difference is found for ALT between groups fed with 500 and 1000 mg/kg body weight of A. nilotica (L.) leaf extract (26.52±1.23 and 30.05±1.38 U/L) and 10 and 20 mg/kg of Ethyl gallate (20.50±0.94 and 24.67±1.13 U/L)^[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

198.17

C₉H₁₀O₅

831-61-8

没食子酸乙酯

Room temperature in continental US; may vary elsewhere.

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

In Vitro: 

DMSO : ≥ 100 mg/mL (504.62 mM)

* “≥” means soluble, but saturation unknown.

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 2.5 mg/mL (12.62 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (12.62 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.5 mg/mL (12.62 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (12.62 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.5 mg/mL (12.62 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (12.62 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Kim WH, et al. Ethyl gallate induces apoptosis of HL-60 cells by promoting the expression of caspases-8, -9, -3, apoptosis-inducing factor and endonuclease G. Int J Mol Sci. 2012;13(9):11912-22.

  [2]. Mohan S, et al. In vitro protection of biological macromolecules against oxidative stress and in vivo toxicity evaluation of Acacia nilotica (L.) and ethyl gallate in rats. BMC Complement Altern Med. 2014 Jul 21;14:257.

The expression of apoptosis-related proteins (caspases-8, -9, -3; AIF; Endo G; Bid; Bax; and Bcl-2) in HL-60 cells is determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of lysates followed by western blotting. For this, HL-60 cells (1.5×10⁶) are treated with 50 μM or 75 μM Ethyl gallate for 6 h, 12 h, or 24 h. Total cell lysates are obtained by resuspending cells in ice-cold radioimmunoprecipitation assay (RIPA) buffer for 30 min followed by centrifugation. Protein concentration is determined using a NanoDrop spectrophotometer. Aliquots of lysates (100 μg protein equivalents) are resolved by 12% SDS-PAGE and transferred onto nitrocellulose membranes^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

HL-60 cells (1×10⁶) are treated with 50 μM or 75 μM Ethyl gallate for 24 h or 48 h at 37°C. Cells are then harvested by centrifugation and fixed in 70% ethanol at 4°C for 24 h. Fixed cells are resuspended in PBS containing 40 μg/mL Propidium iodide (PI), 100 μg/mL RNase A, and 0.1% Triton X-100 and incubated in the dark for 30 min at room temperature. Cell cycle distribution is analyzed by flow cytometry on a FACSCalibur. To investigate apoptotic cells, HL-60 cells (1×10⁶) incubated with different concentration of 50 μM, 75 μM and 100 μM Ethyl gallate for 24 h or 48 h at 37°C, and then DAPI staining is conducted. The cells are photographed using a fluorescence microscopy^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Forty eight female albino Wistar rats of six to eight weeks old are used and divided into eight groups based on their body weights. Group 1 rats serve as control receiving 1.0 mL of the vehicle (0.1% ethanol); Group 2 rats receive A. nilotica (L.) leaf extract (250 mg/kg body weight); Group 3 rats receive A. nilotica (L.) leaf extract (500 mg/kg body weight); Group 4 rats receive A. nilotica (L.) leaf extract (1000 mg/kg body weight); Group 5 rats receive A. nilotica (L.) leaf extract (2000 mg/kg body weight); Group 6 rats receive Ethyl gallate (5 mg/kg body weight); Group 7 rats receive Ethyl gallate (10 mg/kg body weight); Group 8 rats receive Ethyl gallate (20 mg/kg body weight). Body weights are recorded on 0^th and 14^th day for each group and all rats are decapitated after an overnight fast^[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Kim WH, et al. Ethyl gallate induces apoptosis of HL-60 cells by promoting the expression of caspases-8, -9, -3, apoptosis-inducing factor and endonuclease G. Int J Mol Sci. 2012;13(9):11912-22.

  [2]. Mohan S, et al. In vitro protection of biological macromolecules against oxidative stress and in vivo toxicity evaluation of Acacia nilotica (L.) and ethyl gallate in rats. BMC Complement Altern Med. 2014 Jul 21;14:257.