NMS-859 | 赛默飞Alfa aesar官网

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NMS-859 纯度: 98.01%

NMS-859 是一种有效的，共价的 VCP (p97) 抑制剂，细胞中分别在 60 μM 和 1 mM ATP 的条件下，对野生型 VCP 的 IC₅₀ 值分别为 0.37 和 0.36 μM。

NMS-859 Chemical Structure

CAS No. : 1449236-96-7

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥4488	In-stock
2 mg	￥2333	In-stock
5 mg	￥3500	In-stock
10 mg	￥5000	In-stock
50 mg	￥15000	In-stock
100 mg	￥21000	In-stock
200 mg		询价
500 mg		询价

* Please select Quantity before adding items.

NMS-859 相关产品

•相关化合物库:

Covalent Screening Library Plus
Bioactive Compound Library Plus
Cell Cycle/DNA Damage Compound Library
Anti-Cancer Compound Library
Antiviral Compound Library
Anti-Aging Compound Library
Covalent Screening Library
Ubiquitination Compound Library
Endoplasmic Reticulum Stress Compound Library

生物活性

NMS-859 is a potent, covalent VCP (p97) inhibitor, with IC₅₀s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively.

IC₅₀ & Target

IC50: 360 nM (Cellular p97, 1 mM ATP), 370 nM (Cellular p97, 60 μM ATP)^[1]

体外研究
(In Vitro)

NMS-859 is a potent VCP inhibitor, with IC₅₀s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively. NMS-859 shows very weak inhibitory activity against VCP^C522T. NMS-859 also suppresses the proliferation of cells, with IC₅₀s of 3.5 μM and 3.0 μM in HCT116 and HeLa cell lines, respectively^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

349.79

Formula

C₁₅H₁₂ClN₃O₃S

CAS 号

1449236-96-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : ≥ 42 mg/mL (120.07 mM)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8589 mL	14.2943 mL	28.5886 mL
5 mM	0.5718 mL	2.8589 mL	5.7177 mL
10 mM	0.2859 mL	1.4294 mL	2.8589 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

Cell Assay ^[1]	Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with NMS-859 (eight dilution points, in duplicate) and incubated for an additional 72 h at 37°C under a 5% CO₂ atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase-based assay as a measure of cell viability. IC₅₀ values are calculated using the percentage of growth of treated cells versus the untreated control^[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

Cell Assay
^[1]

Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with NMS-859 (eight dilution points, in duplicate) and incubated for an additional 72 h at 37°C under a 5% CO₂ atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase-based assay as a measure of cell viability. IC₅₀ values are calculated using the percentage of growth of treated cells versus the untreated control^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

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