Methotrexate disodium(Synonyms: Amethopterin disodium; CL14377 disodium; WR19039 disodium)

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Methotrexate disodium (Synonyms: Amethopterin disodium; CL14377 disodium; WR19039 disodium) 纯度: 98.26%

Methotrexate (Amethopterin) disodium 是一种抗代谢 (antimetabolite) 和抗叶酸(antifolate) 剂，可抑制二氢叶酸还原酶，从而防止叶酸转化为四氢叶酸并抑制 DNA 合成。Methotrexate disodium 也是一种免疫抑制剂和抗肿瘤剂，用于类风湿关节炎和研究多种癌症 (如急性淋巴细胞白血病)。

Methotrexate disodium(Synonyms: Amethopterin disodium; CL14377 disodium; WR19039 disodium)

Methotrexate disodium Chemical Structure

CAS No. : 7413-34-5

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in Water ￥660 In-stock
100 mg ￥600 In-stock
500 mg ￥1560 In-stock
1 g   询价  
5 g   询价  

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生物活性

Methotrexate (Amethopterin) disodium, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate disodium, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].

IC50 & Target

Traditional Cytotoxic Agents

 

体内研究
(In Vivo)

Methotrexate (Amethopterin) disodium reduces thymus and spleen indices of mice. Methotrexate disodium markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate exposure on indices of thymus and spleens in mice[2].
Methotrexate (MTX) disodium (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund’s complete adjuvant-induced arthritis. The combination of Methotrexate disodium (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[4].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

498.40

Formula

C20H20N8Na2O5
CAS 号

7413-34-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro: 

H2O : 50 mg/mL (100.32 mM; Need ultrasonic)

DMSO : 5 mg/mL (10.03 mM; ultrasonic and warming and adjust pH to 5 with HCl and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0064 mL 10.0321 mL 20.0642 mL
5 mM 0.4013 mL 2.0064 mL 4.0128 mL
10 mM 0.2006 mL 1.0032 mL 2.0064 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73.

    [2]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.

    [3]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.

    [4]. Banji D, et al. Evaluation of the concomitant use of methotrexate and curcumin on Freund’s complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol. 2011;43(5):546-550.

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