Terrein(Synonyms: 土曲霉酮)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Terrein (Synonyms: 土曲霉酮)

Terrein 是一种黑色素生成抑制剂。Terrein 诱导乳腺癌细胞凋亡 (apoptosis)。Terrein是铜绿假单胞菌 (Pseudomonas aeruginosa) 中的群体感应和 c-di-GMP 抑制剂。

Terrein(Synonyms: 土曲霉酮)

Terrein Chemical Structure

CAS No. : 582-46-7

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生物活性

Terrein is a melanogenesis inhibitor[1]. Terrein induces apoptosis in breast cancer cell lines [2]. Terrein is an inhibitor of quorum sensing and c-di-GMP in Pseudomonas aeruginosa[3].

体外研究
(In Vitro)

Treatment of Mel-Ab cells with Terrein (10-100 µM) for 4 days significantly reduces melanin levels in a dose-dependent manner. Terrein reduces melanin synthesis by reducing tyrosinase production via ERK activation[1].
Terrein (5-500 µM; 24 and 48 hours) reduces the viability of these cells in concentration- and time-dependent manners[2].
Terrein (150, 250, 500µM; 24 hours) induces programmed cell death in both invasive and non-invasive breast cancer cell lines. Thus, the optimal non-toxic concentrations of Terrein (25 and 75 µM) were used for subsequent experiments[2].
Terrein (25 and 75 µM) significantly decrease the mRNA levels of both MMP-2 and MMP-9 in MDA-MB-231 cells. Terrein (75 µM) significantly decrease the mRNA levels of both MMP-2 and MMP-9 in MCF-7 cells[2].
Terrein (75 µM) inhibits the expression of RhoA, RhoB, p-Rac1 in MCF-7 cells. Terrein (75 µM) inhibits the expression of RhoB, p-Rac1 in MDA-MB-231 cells[2].
Terrein (10, 30, and 100 μM) inhibits the production of virulence factors such as elastase, pyocyanin, and rhamnolipid, as well as biofilm formation in P. aeruginosa PAO1 and PA14 strains[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 5, 25, 50, 75, 100, 150, 250, 500 µM
Incubation Time: 24 and 48 hours
Result: The IC50s for MCF-7 and MDA-MB-231 cells after 24 h of incubation were 2.34 mM and 700 µM, respectively. After 48 h of incubation, the IC50s are 244.3 µM for MCF-7 and 244.5 µM for MDA-MB-231 cells.

Apoptosis Analysis[2]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 25, 75, 150, 250, 500 µM
Incubation Time: 24 hours
Result: The percentages of apoptotic cells after 25 and 75 µM treatment were 7.52 and 5.82%, respectively for MCF-7 cells (9.94% for the control), and 15.77 and 15.82%, respectively for MDA-MB-231 cells (8.79% for the control).

RT-PCR[2]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 25, 75 µM
Incubation Time:
Result: Decreased the mRNA levels of both MMP-2 and MMP-9 in a concentration-dependent manner.

Western Blot Analysis[2]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 75 µM
Incubation Time: 6, 12 and 24 hours
Result: RhoA and RhoB protein levels were significantly decreased after 6 h of 75 µM treatment in MCF-7 cells.
Phosphorylated (p)-Rac1 was also slightly decreased at 12 h. However, RhoC and Cdc42 levels remained unchanged. RhoB protein levels and Rac1 phosphorylation were also decreased in MDA-MB-231 cells at 12 and 24 h, respectively.

分子量

154.16

Formula

C8H10O3

CAS 号

582-46-7

中文名称

土曲霉酮

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. S-H Park, et al. Terrein: A New Melanogenesis Inhibitor and Its Mechanism. Cell Mol Life Sci. 2004 Nov;61(22):2878-85.

    [2]. Anongnard Kasorn, et al. Terrein Inhibits Migration of Human Breast Cancer Cells via Inhibition of the Rho and Rac Signaling Pathways. Oncol Rep. 2018 Mar;39(3):1378-1386.

    [3]. Bomin Kim, et al. Terrein Is an Inhibitor of Quorum Sensing and c-di-GMP in Pseudomonas Aeruginosa: A Connection Between Quorum Sensing and c-di-GMP. Sci Rep. 2018 Jun 5;8(1):8617.

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