Ilexgenin A

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Ilexgenin A  纯度: 98.00%

Ilexgenin A 是从海南冬青中提取的一种五环三萜类化合物。Ilexgenin A 可用于炎症和癌症研究。

Ilexgenin A

Ilexgenin A Chemical Structure

CAS No. : 108524-94-3

规格 价格 是否有货 数量
1 mg ¥900 In-stock
5 mg ¥1900 In-stock
10 mg ¥2900 In-stock
25 mg ¥4400 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Ilexgenin A 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library

生物活性

Ilexgenin A is a pentacyclic triterpenoid, which extracted from Ilex hainanensis Merr. Ilexgenin A can be used for the research of inflammation and cancer[1].

体外研究
(In Vitro)

Ilexgenin A (0~100 μM; 24 hours; HT 29 and HCT 116 cells) effectively decreases cell viability in HT 29 and HCT 116 cells[1].
Ilexgenin A (12.5~50 μM; 24 hours; HT 29 cells) downregulates the expression of SREBP-1, inhibits the translocation of SREBP-1 to the nucleus and decreases the content of TG[1].
Ilexgenin A (12.5~50 μM; HT 29 and HCT 116 cells) induces cell cycle arrest at G1 phase in colon cancer cells[1].
Ilexgenin A (12.5~50 μM; 24 hours; HCT 116 cells) modulates lipid metabolism. Ilexgenin A (50 μM; 24 hours; HT 29 and HCT 116 cells) regulates SREBP-1 by inhibiting HIF1α[1].
Ilexgenin A inhibits fatty acids synthesis[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HT 29 and HCT 116 cells
Concentration: 0~100 μM
Incubation Time: 24 hours
Result: Effectively decreased cell viability in HT 29 and HCT 116 cells.

Western Blot Analysis[1]

Cell Line: HT 29 cells
Concentration: 12.5~50 μM
Incubation Time: 24 hours
Result: Downregulated the expression of SREBP-1.

RT-PCR[1]

Cell Line: HT 29 cells
Concentration: 12.5~50 μM
Incubation Time: 24 hours
Result: Decreased the content of TG.

Immunofluorescence[1]

Cell Line: HT 29 cells
Concentration: 12.5~50 μM
Incubation Time: 24 hours
Result: Inhibited the translocation of SREBP-1 to the nucleus.

体内研究
(In Vivo)

Ilexgenin A (20 mg/kg) ameliorates the AOM/DSS induced carcinogenesis. Ilexgenin A can regulate lipid metabolism. Ilexgenin A decreases the expression of SREBP-1 and HIF 1α in AOM/DSS mice[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

502.68

Formula

C30H46O6

CAS 号

108524-94-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL (198.93 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9893 mL 9.9467 mL 19.8934 mL
5 mM 0.3979 mL 1.9893 mL 3.9787 mL
10 mM 0.1989 mL 0.9947 mL 1.9893 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (4.97 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.97 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.97 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.97 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Zhang L, et al. Ilexgenin A prevents early colonic carcinogenesis and reprogramed lipid metabolism through HIF1α/SREBP-1. Phytomedicine. 2019;63:153011.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务