Ilexgenin A is a pentacyclic triterpenoid, which extracted from Ilex hainanensis Merr. Ilexgenin A can be used for the research of inflammation and cancer^[1].

Ilexgenin A (0~100 μM; 24 hours; HT 29 and HCT 116 cells) effectively decreases cell viability in HT 29 and HCT 116 cells^[1].
Ilexgenin A (12.5~50 μM; 24 hours; HT 29 cells) downregulates the expression of SREBP-1, inhibits the translocation of SREBP-1 to the nucleus and decreases the content of TG^[1].
Ilexgenin A (12.5~50 μM; HT 29 and HCT 116 cells) induces cell cycle arrest at G1 phase in colon cancer cells^[1].
Ilexgenin A (12.5~50 μM; 24 hours; HCT 116 cells) modulates lipid metabolism. Ilexgenin A (50 μM; 24 hours; HT 29 and HCT 116 cells) regulates SREBP-1 by inhibiting HIF1α^[1].
Ilexgenin A inhibits fatty acids synthesis^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Ilexgenin A (20 mg/kg) ameliorates the AOM/DSS induced carcinogenesis. Ilexgenin A can regulate lipid metabolism. Ilexgenin A decreases the expression of SREBP-1 and HIF 1α in AOM/DSS mice^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

502.68

C₃₀H₄₆O₆

108524-94-3

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DMSO : 100 mg/mL (198.93 mM; Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: 2.5 mg/mL (4.97 mM); Suspended solution; Need ultrasonic

  此方案可获得 2.5 mg/mL (4.97 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.5 mg/mL (4.97 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (4.97 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Zhang L, et al. Ilexgenin A prevents early colonic carcinogenesis and reprogramed lipid metabolism through HIF1α/SREBP-1. Phytomedicine. 2019;63:153011.