Autophagy inducer 3

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Autophagy inducer 3 

Autophagy inducer 3 具有细胞自噬 (autophagy) 诱导活性。Autophagy inducer 3 能强烈地诱导不同癌细胞死亡，而不影响正常细胞。Autophagy inducer 3 通过形成特征性的自噬空泡、形成 LC3 点状体、上调自噬标志蛋白 Beclin 和 Atg 家族蛋白等，来诱导细胞自噬。

Autophagy inducer 3

Autophagy inducer 3 Chemical Structure

CAS No. : 2691054-63-2

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生物活性

Autophagy inducer 3 has autophagy induced activity. Autophagy inducer 3 possesses robust autophagic cell death in diverse cancer cells sparing normal counterpart. Autophagy inducer 3 induces lethal autophagy by formation of characteristic autophagic vacuoles, LC3 puncta formation, upregulation of signature autophagy markers like Beclin and Atg family proteins[1].

IC50 & Target

Autophagy[1]
体外研究
(In Vitro)

Autophagy inducer 3 (compound 26b) (10 μM; 48 hours) effectively inhibits cell growth in diverse breast, lung and colon cancer cell lines[1].
Autophagy inducer 3 (0-10 μM; 48 hours) exhibits potent antiproliferative activity in COLO-205, LOVO, HT-29, DLD-1, SW48 and SW-620 with IC50s of 2.03 μM, 3.33 μM, 4.15 μM, 4.46 μM, 3.14 μM, 1.86 μM, respectively; and shows low cytotoxicity in normal human colon fibroblast CCD-18Co with IC50 over 10 μM[1].
Autophagy inducer 3 (7.5 μM; 18 hours) promotes non apoptotic cell death in DLD-1 cells by cellular granularity through vacuole formation and increase Annexin-V positive cells as well as PARP cleavage; and robustly induces the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: MCF-7, MDA-MB-231, DLD-1, HT-29, A549 and NCI-H358[1]
Concentration: 10 μM
Incubation Time: 48 hours
Result: Effectively inhibited cell growth at 10 μM dose in diverse cancer cell lines with the inhibition rates of 89.28~97.66%.

Cell Autophagy Assay

Cell Line: DLD-1[1]
Concentration: 7.5 μM
Incubation Time: 18 hours
Result: Promoted intracytoplasmic vacuole accumulation in colon cancer (DLD-1) cells; and robustly induced the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7.

分子量

377.60

Formula

C24H43NO2
CAS 号

2691054-63-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Ganesher A, et al. New Spisulosine Derivative promotes robust autophagic response to cancer cells. Eur J Med Chem. 2020;188:112011.

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