N-ethylmaleimide, NEM

Description:

N-ethylmaleimide (NEM) is a water soluble, thiol reactive maleimide that can be used to modify protein cysteine residues. Maleimide reacts with sulfhydryl groups at pH 6.5~7.5 to form stable thioether bonds. NEM has been used for protein Acyl-RAC assay. N-ethylmaleimide is a sulfhydryl alkylating reagent that inhibits H+-ATPase and suppresses the short circuit current (IC50 = 22 µM) in pancreatic duct cells. N-ethylmaleimide Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei. NEM stimulates arachidonic acid release through activation of PLA2 in endothelial cells. As a commonly used sulfhydryl alkylating reagent, NEM has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei.

Physical Properties:

• Appearance: Off-white/faint yellow solid
• Mol Wt: 125.13
• CAS No: 128-53-0
• Purity: >98%

Storage Conditions:

• Store at -20 ⁰C.