Grape seed extract is a natural product, with anti-inflammatory and anti-proliferative effects. Grape seed extract shows inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase. Grape seed extract induces apoptotic in human colorectal cancer cells[1][2][3].
体外研究 (In Vitro)
Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells[3]. Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells[3]. Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay[3]
Cell Line:
HT29 cells, LoVo cells
Concentration:
25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time:
12 hours, 24 hours, 48 hours
Result:
Inhibited cell growth in a dose- and a time-dependent manner.
Cell Cycle Analysis[3]
Cell Line:
HT29 cells, LoVo cells
Concentration:
25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time:
12 hours, 24 hours, 48 hours
Result:
Induced cell cycle arrest (a marked increase in Cip1/p21protein level and a decrease in G 1 phase^associated cyclins and cyclin-dependentkinases).
Western Blot Analysis[3]
Cell Line:
HT29 cells, LoVo cells
Concentration:
25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time:
12 hours, 24 hours
Result:
Modulates the protein levels of CDKIs, CDKs, and cyclins.
Apoptosis Analysis[3]
Cell Line:
HT29 cells, LoVo cells
Concentration:
25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time:
24 hours
Result:
Showed a significant dose-dependent increase in apoptotic cell population in HT29 cells at 50 μg/mL and 100 μg/mL; all three Concentrations caused a dose-dependent increase in apoptotic cells.
体内研究 (In Vivo)
Grape seed extract (200 mg/kg; i.g.; 5 days/wk; for 8 weeks) inhibits HT29 colon carcinoma tumor xenograft growth in athymic nude mice[3]. Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors[3]. Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Athymic male nu/nu mice, with human colon carcinoma HT29 tumor xenograft[3]
Dosage:
200 mg/kg
Administration:
Oral gavage, 5 days/wk, for 8 weeks
Result:
Showed time-dependent inhibition of tumor growth.
Clinical Trial
CAS 号
84929-27-1
中文名称
葡萄籽提取物； 葡萄籽精华； 葡萄籽萃取； 葡萄籽萃取物
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:
DMSO : 6 mg/mL (Need ultrasonic)
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
[1]. The effects of grape seed extract (Vitis vinifera) supplement on inflammatory markers, neuropeptide Y, anthropometric measures, and appetite in obese or overweight individuals: A randomized clinical trial.
[2]. Inhibitory effects of grape seed extract on lipases. Nutrition. 2003 Oct;19(10):876-9.
[3]. Manjinder Kaur, et al. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells. Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):6194-202.
Ginger extract exhibits anti-cancer, anti-inflammatory and chemotherapeutic effects in vivo[1].
体内研究 (In Vivo)
Ginger extract (oral gavage; 100 mg/kg; 8 weeks) reduces the incidence of liver neoplasms in rats, it is able to block the elevated expression of NF-κB in liver cancer-induced rats[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Male Wistar rat[1]
Dosage:
100 mg/kg
Administration:
Oral gavage; 8 weeks
Result:
Exhibits anti-cancer effects in the liver cancer-induced group (CDE).
Clinical Trial
CAS 号
84696-15-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
参考文献
[1]. Shafina Hanim Mohd Habib, et al. Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats. Clinics (Sao Paulo). 2008 Dec;63(6):807-13.
Ginger extract exhibits anti-cancer, anti-inflammatory and chemotherapeutic effects in vivo[1].
体内研究 (In Vivo)
Ginger extract (oral gavage; 100 mg/kg; 8 weeks) reduces the incidence of liver neoplasms in rats, it is able to block the elevated expression of NF-κB in liver cancer-induced rats[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Male Wistar rat[1]
Dosage:
100 mg/kg
Administration:
Oral gavage; 8 weeks
Result:
Exhibits anti-cancer effects in the liver cancer-induced group (CDE).
Clinical Trial
CAS 号
84696-15-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
参考文献
[1]. Shafina Hanim Mohd Habib, et al. Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats. Clinics (Sao Paulo). 2008 Dec;63(6):807-13.
Ginger extract exhibits anti-cancer, anti-inflammatory and chemotherapeutic effects in vivo[1].
体内研究 (In Vivo)
Ginger extract (oral gavage; 100 mg/kg; 8 weeks) reduces the incidence of liver neoplasms in rats, it is able to block the elevated expression of NF-κB in liver cancer-induced rats[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Male Wistar rat[1]
Dosage:
100 mg/kg
Administration:
Oral gavage; 8 weeks
Result:
Exhibits anti-cancer effects in the liver cancer-induced group (CDE).
Clinical Trial
CAS 号
84696-15-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
参考文献
[1]. Shafina Hanim Mohd Habib, et al. Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats. Clinics (Sao Paulo). 2008 Dec;63(6):807-13.
