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PI3K/Akt/mTOR-IN-2
PI3K/Akt/mTOR-IN-2 是一种有效的 PI3K/AKT/mTOR 抑制剂。PI3K/Akt/mTOR-IN-2 具有抗癌作用，并对 MDA-MB-231 细胞具有选择性，IC50 为 2.29 μM。PI3K/Akt/mTOR-IN-2 可诱导癌细胞周期阻滞和细胞凋亡 (apoptosis)。
PI3K/Akt/mTOR-IN-2 Chemical Structure
CAS No. : 2757804-89-8
| 规格 |
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是否有货 |
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| 100 mg |
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询价 |
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| 250 mg |
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询价 |
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| 500 mg |
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询价 |
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* Please select Quantity before adding items.
| 生物活性 |
PI3K/Akt/mTOR-IN-2 is a PI3K/AKT/mTOR pathway inhibitor. PI3K/Akt/mTOR-IN-2 possess anti-cancer effects and selectivity against MDA-MB-231 cells with IC50 value of 2.29 μM. PI3K/Akt/mTOR-IN-2 can induce cancer cell cycle arrest and apoptosis[1].
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IC50 & Target |
IC50: 2.29 μM (PI3K/AKT/mTOR) in MDA-MB-231[1]
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体外研究
(In Vitro) |
PI3K/Akt/mTOR-IN-2 (compound 23) (0.5 – 100 μM; 72 hours) exhibits effective anti-cancer activity with IC50s of 2.29 – 24.63 μM, of which, IC50 in MDA-MB-231 is 2.29 μM[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24, 48 and 72 hours) induces apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 48 hours) increases the expression of Bax, and decreases the expression of Bcl-2 in MDA-MB-231 cells[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces mitochondria-dependent apoptosis in MDA-MB-231 cells through disruption of MMP, accumulation of ROS, depletion of GSH and elevation of intracellular Ca2+[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay
| Cell Line: |
PC3, BGC-823, A549, MCF-7, MDA-MB-231 cells and MCF-10A cells[1] |
| Concentration: |
0.5 – 100 μM |
| Incubation Time: |
72 hours |
| Result: |
Exhibited effective anti-cancer activity with IC50s of 2.29 – 24.63 μM, of which, IC50 in MDA-MB-231 was 2.29 μM. |
Cell Cycle Analysis
| Cell Line: |
MDA-MB-231[1] |
| Concentration: |
1 μM, 2 μM and 4 μM |
| Incubation Time: |
24 hours |
| Result: |
Induced growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1. |
Apoptosis Analysis
| Cell Line: |
MDA-MB-231[1] |
| Concentration: |
1 μM, 2 μM and 4 μM |
| Incubation Time: |
24, 48 and 72 hours |
| Result: |
Induced apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners. |
Western Blot Analysis
| Cell Line: |
MDA-MB-231[1] |
| Concentration: |
1 μM, 2 μM and 4 μM |
| Incubation Time: |
48 hours |
| Result: |
Increased the expression of Bax, and decreased the expression of Bcl-2 |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 参考文献 |
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[1]. Qin J, Sun X, Ma Y, et al. Design, synthesis and biological evaluation of novel 1,3,4,9-tetrahydropyrano[3,4-b]indoles as potential treatment of triple negative breast cancer by suppressing PI3K/AKT/mTOR pathway [published online ahead of print, 2021 Dec 31]. Bioorg Med Chem. 2021;55:116594.
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