SAG hydrochloride 是一种有效的 Smoothened (Smo) 受体激动剂 (EC50=3 nM; Kd=59 nM)。SAG hydrochloride 可激活 Hedgehog 信号通路并抵消 Cyclopamine (HY-17024) 对 Smo 的抑制作用。
SAG hydrochloride Chemical Structure
CAS No. : 2095432-58-7
规格
价格
是否有货
数量
10 mM * 1 mL in DMSO
¥2085
In-stock
1 mg
¥600
In-stock
5 mg
¥1800
In-stock
10 mg
¥2900
In-stock
25 mg
¥5800
In-stock
50 mg
¥9800
In-stock
100 mg
询价
200 mg
询价
* Please select Quantity before adding items.
SAG hydrochloride 相关产品
•相关化合物库:
Bioactive Compound Library Plus
Stem Cell Signaling Compound Library
Wnt/Hedgehog/Notch Compound Library
Anti-Cancer Compound Library
Anti-Aging Compound Library
Reprogramming Compound Library
Anti-Pancreatic Cancer Compound Library
生物活性
SAG hydrochloride is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG hydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].
IC50 & Target
EC50: 3 nM (Smo)[1]
体外研究 (In Vitro)
SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations[1]. SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex[1]. SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin[2]. SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells[3]. SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells[3]. SAG (250 nM; 24 h) increases MDAMB231 cells migration[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice[4]. SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice[5].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Pregnant C57BL/6J mice[5]
Dosage:
15, 17, 20 mg/kg
Administration:
A single i.p.
Result:
Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.
分子量
526.52
Formula
C28H29Cl2N3OS
CAS 号
2095432-58-7
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.
[2]. Stanton BZ, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6.
[3]. Guerrini G, et, al. Inhibition of smoothened in breast cancer cells reduces CAXII expression and cell migration. J Cell Physiol. 2018 Dec; 233(12): 9799-9811.
[4]. Lee S, et al. Combining Smoothened Agonist (SAG) and NEL-like protein-1 (NELL-1) Enhances Bone Healing. Plast Reconstr Surg. 2017 Feb 13.
[5]. Fish EW, et al. Preaxial polydactyly following early gestational exposure to the smoothened agonist, SAG, in C57BL/6J mice. Birth Defects Res A Clin Mol Teratol. 2016 Nov 1.
SAG 是一种有效的 Smoothened (Smo) 受体激动剂 (EC50=3 nM; Kd=59 nM)。SAG 可激活 Hedgehog 信号通路并抵消 Cyclopamine (HY-17024) 对 Smo 的抑制作用。
SAG Chemical Structure
CAS No. : 912545-86-9
规格
价格
是否有货
数量
10 mM * 1 mL in DMSO
¥1941
In-stock
1 mg
¥600
In-stock
5 mg
¥1800
In-stock
10 mg
¥2900
In-stock
25 mg
¥5800
In-stock
50 mg
¥9800
In-stock
100 mg
询价
200 mg
询价
* Please select Quantity before adding items.
SAG 相关产品
•相关化合物库:
Bioactive Compound Library Plus
Stem Cell Signaling Compound Library
Wnt/Hedgehog/Notch Compound Library
Anti-Cancer Compound Library
Anti-Aging Compound Library
Reprogramming Compound Library
Anti-Pancreatic Cancer Compound Library
Targeted Diversity Library
生物活性
SAG is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].
IC50 & Target
EC50: 3 nM (Smo)[1]
体外研究 (In Vitro)
SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations[1]. SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex[1]. SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin[2]. SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells[3]. SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells[3]. SAG (250 nM; 24 h) increases MDAMB231 cells migration[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice[4]. SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice[5].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Pregnant C57BL/6J mice[5]
Dosage:
15, 17, 20 mg/kg
Administration:
A single i.p.
Result:
Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.
分子量
490.06
Formula
C28H28ClN3OS
CAS 号
912545-86-9
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.
[2]. Stanton BZ, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6.
[3]. Guerrini G, et, al. Inhibition of smoothened in breast cancer cells reduces CAXII expression and cell migration. J Cell Physiol. 2018 Dec; 233(12): 9799-9811.
[4]. Lee S, et al. Combining Smoothened Agonist (SAG) and NEL-like protein-1 (NELL-1) Enhances Bone Healing. Plast Reconstr Surg. 2017 Feb 13
[5]. Fish EW, et al. Preaxial polydactyly following early gestational exposure to the smoothened agonist, SAG, in C57BL/6J mice. Birth Defects Res A Clin Mol Teratol. 2016 Nov 1
SAG dihydrochloride 是一种有效的 Smoothened (Smo) 受体激动剂 (EC50=3 nM; Kd=59 nM)。SAG dihydrochloride 可激活 Hedgehog 信号通路并抵消 Cyclopamine (HY-17024) 对 Smo 的抑制作用。
SAG dihydrochloride Chemical Structure
规格
价格
是否有货
数量
10 mM * 1 mL in DMSO
¥2910
In-stock
1 mg
¥750
In-stock
5 mg
¥2350
In-stock
10 mg
¥3750
In-stock
50 mg
¥8500
In-stock
100 mg
询价
200 mg
询价
* Please select Quantity before adding items.
SAG dihydrochloride 相关产品
•相关化合物库:
Bioactive Compound Library Plus
Stem Cell Signaling Compound Library
Wnt/Hedgehog/Notch Compound Library
Anti-Cancer Compound Library
Anti-Aging Compound Library
Reprogramming Compound Library
Anti-Pancreatic Cancer Compound Library
生物活性
SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].
IC50 & Target
EC50: 3 nM (Smo)[1]
体外研究 (In Vitro)
SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations[1]. SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex[1]. SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin[2]. SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells[3]. SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells[3]. SAG (250 nM; 24 h) increases MDAMB231 cells migration[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice[4]. SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice[5].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Pregnant C57BL/6J mice[5]
Dosage:
15, 17, 20 mg/kg
Administration:
A single i.p.
Result:
Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.
分子量
562.98
Formula
C28H30Cl3N3OS
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.
[2]. Stanton BZ, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6.
[3]. Guerrini G, et, al. Inhibition of smoothened in breast cancer cells reduces CAXII expression and cell migration. J Cell Physiol. 2018 Dec; 233(12): 9799-9811.
[4]. Lee S, et al. Combining Smoothened Agonist (SAG) and NEL-like protein-1 (NELL-1) Enhances Bone Healing. Plast Reconstr Surg. 2017 Feb 13.
[5]. Fish EW, et al. Preaxial polydactyly following early gestational exposure to the smoothened agonist, SAG, in C57BL/6J mice. Birth Defects Res A Clin Mol Teratol. 2016 Nov 1.