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| 名称 | Guanylin, rat, mouse |
| 编码 | |
| 别名 | Guanylin, rat, mouse |
| 纯度 | 80%，90%，95%，98%，99% |
| 重量 | 1mg,5mg,10mg,50mg,100mg,1g |
| 序列（单字母缩写） | PNTCEICAYAACTGC (C4&C12 bridge , C7&C15 bridge) |
| 序列（三字母缩写） | Pro-Asn-Thr-Cys-Glu-Ile-Cys-Ala-Tyr-Ala-Ala-Cys-Thr-Gly-Cys(Cys4&Cys12 bridge , Cys7&Cys15 bridge) |
| 基本描述 | A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity. |
| 溶解度 | |
| 分子量 | 1515.7 |
| 化学式 | C60H90N16O22S4 |
| 存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
| 注释 | |
| Documents |  |
| Figures |  |
| Reference | R.C. Wiegand et al., BBRC, 185, 812 (1992) |
| C端 | |
| N端 | |
| 化学桥 | (Cys4&Cys12 bridge , Cys7&Cys15 bridge) |