Ziyuglycoside II(Synonyms: 地榆皂苷II)

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Ziyuglycoside II (Synonyms: 地榆皂苷II) 纯度: 99.77%

Ziyuglycoside II 是从 Sanguisorba officinalis L. 中提取的三萜皂苷化合物。Ziyuglycoside II 诱导活性氧 (ROS) 产生和凋亡 (apoptosis)。 具有抗炎和抗癌作用。

Ziyuglycoside II(Synonyms: 地榆皂苷II)

Ziyuglycoside II Chemical Structure

CAS No. : 35286-59-0

规格 价格 是否有货 数量
5 mg ￥1080 In-stock
10 mg ￥1600 In-stock
50 mg   询价  
100 mg   询价  

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Ziyuglycoside II 相关产品

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生物活性

Ziyuglycoside II is a triterpenoid saponin compound extracted from Sanguisorba officinalis L.. Ziyuglycoside II induces reactive oxygen species (ROS) production and apoptosis. Anti-inflammation and anti-cancer effect[1].

体外研究
(In Vitro)

Ziyuglycoside II (10-60 μM; 24 h and 48 h) inhibits MDA-MB-435 cells growth in a dose-dependent manner. The IC50 of Ziyuglycoside II at 24 h and 48 h is 5.92 μM and 4.74 μM, respectively[1].
Ziyuglycoside II (5-25 μM) induces G0/G1 and S phase arrest in MDA-MB-435 cells at 24 h[1].
Ziyuglycoside II (5-25 μM; 24 hours) significantly increases apoptotic rate of MDA-MB-435 cells[1].
Ziyuglycoside II (5-25 μM; 24 hours) increases expressions of both p53 and p21 in MDA-MB-435 cells, which effect is dose-dependent[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: MDA-MB-435 cells
Concentration: 10, 20, 30, 40, 50, 60 μM
Incubation Time: 24 hours and 48 hours
Result: The IC50 at 24 h and 48 h was 5.92 μM and 4.74 μM, respectively.

Cell Cycle Analysis[1]

Cell Line: MDA-MB-435 cells
Concentration: 5, 10, 25 μM
Incubation Time: 24 hours
Result: Induced G0/G1 and S phase arrest.

Apoptosis Analysis[1]

Cell Line: MDA-MB-435 cells
Concentration: 5, 10, 25 μM
Incubation Time: 24 hours
Result: The apoptotic rate was significantly increased in comparison to that of the control.

Western Blot Analysis[1]

Cell Line: MDA-MB-435 cells
Concentration: 5, 10, 25 μM
Incubation Time: 24 hours
Result: Treatment resulted in increased expressions of both p53 and p21.

分子量

604.81

Formula

C35H56O8
CAS 号

35286-59-0
中文名称

地榆皂苷II
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

DMSO : 50 mg/mL (82.67 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6534 mL 8.2671 mL 16.5341 mL
5 mM 0.3307 mL 1.6534 mL 3.3068 mL
10 mM 0.1653 mL 0.8267 mL 1.6534 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (4.13 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.13 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.13 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.13 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Zhu X, et al. Ziyuglycoside II inhibits the growth of human breast carcinoma MDA-MB-435 cells via cell cycle arrest and induction of apoptosis through the mitochondria dependent pathway. Int J Mol Sci. 2013 Sep 3;14(9):18041-55.

    [2]. Zhu X, et al. Ziyuglycoside II induces cell cycle arrest and apoptosis through activation of ROS/JNK pathway in human breast cancer cells. Toxicol Lett. 2014 May 16;227(1):65-73.

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