Nitazoxanide(Synonyms: 硝唑尼特; NTZ; NSC 697855)

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Nitazoxanide (Synonyms: 硝唑尼特; NTZ; NSC 697855) 纯度: 98.35%

Nitazoxanide (NTZ) 是一种广谱驱虫剂 (anthelmintic)，对感染动物和人类的各种蠕虫、原生动物和肠道细菌都具有作用活性。 Nitazoxanide 在无菌培养中抑制 Giardia lamblia 滋养体增殖，IC50 为 2.4 μM。Nitazoxanide 可用于寄生虫性 (parasitic) 胃肠炎的研究。Nitazoxanide还具有抗病毒特性。 Nitazoxanide 在小鼠模型中显示出抗日本脑炎病毒 (JEV) 活性。

Nitazoxanide(Synonyms: 硝唑尼特; NTZ; NSC 697855)

Nitazoxanide Chemical Structure

CAS No. : 55981-09-4

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10 mM * 1 mL in DMSO ￥500 In-stock
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100 mg ￥1989 In-stock
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生物活性

Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1]. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model[2].

体外研究
(In Vitro)

Giardia lamblia, a flagellated protozoan, is the most common causative agent of persistent diarrhea worldwide[1].
Nitazoxanide exhibits effect on G. lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Human cancer colon Caco2 cells were incubated with increasing numbers of Giardia lamblia trophozoites (103 to 106 parasites per well)
Concentration: 30 μM
Incubation Time: 24 hours
Result: 70 to 90% of the trophozoites remained attached to the Caco2 cells for a period of 24 to 48 h in the absence of Nitazoxanide and at an initial inoculum density of 105 parasites per well.
The number of parasites still attached to Caco2 cells after 24 h decreased to less than 20% of the control value in the presence of 30 μM Nitazoxanide with an inoculum density of 105 trophozoites.

体内研究
(In Vivo)

Nitazoxanide exhibits a wide spectrum of in vivo activity against a broad spectrum of intestinal parasites, such as Giardia lamblia, Entamoeba histolytica, Trichomonas vaginalis, the apicomplexan Cryptosporidium parvum, and enteric bacteria infecting animals and humans[1].
Nitazoxanide (50, 75 or 100 mg/kg/day; administered daily by intragastric for up to 25 days) reduces the mortality of Japanese encephalitis virus (JEV) strain-infected mice and protected mice from a lethal dose challenge of JEV[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Three-week old female Chinese Kunming mice (12–14 g body weight) were infected intraperitoneally with JEV[2]
Dosage: 50, 75 or 100 mg/kg/day
Administration: Administered intragastrically by gavage
Result: 50 mg/kg/day, 75 mg/kg/day and 100 mg/kg/day led to 30%, 70% and 90% mice survival, respectively.

分子量

307.28

Formula

C12H9N3O5S
CAS 号

55981-09-4
中文名称

硝唑尼特
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL (325.44 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2544 mL 16.2718 mL 32.5436 mL
5 mM 0.6509 mL 3.2544 mL 6.5087 mL
10 mM 0.3254 mL 1.6272 mL 3.2544 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 3.25 mg/mL (10.58 mM); Clear solution

    此方案可获得 ≥ 3.25 mg/mL (10.58 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Rossignol JF, et al. Thiazolides, a new class of anti-influenza molecules targeting viral hemagglutinin at the post-translational level. J Biol Chem. 2009 Oct 23;284(43):29798-808.

    [2]. Zixue Shi, et al. Nitazoxanide inhibits the replication of Japanese encephalitis virus in cultured cells and in a mouse model. Virol J. 2014 Jan 23;11:10.

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