Berberine chloride hydrate(Synonyms: 盐酸黄连素水合物; Natural Yellow 18 chloride hydrate)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Berberine chloride hydrate (Synonyms: 盐酸黄连素水合物; Natural Yellow 18 chloride hydrate) 纯度: 99.84%

Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) 是一种生物碱，常用作抗生素。Berberine chloride hydrate 诱导活性氧 (ROS) 生成并抑制 DNA 拓扑异构酶 (topoisomerase)。抗肿瘤特性。

Berberine chloride hydrate(Synonyms: 盐酸黄连素水合物; Natural Yellow 18 chloride hydrate)

Berberine chloride hydrate Chemical Structure

CAS No. : 68030-18-2

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ￥990 In-stock
100 mg ￥660 In-stock
1 g ￥1500 In-stock
5 g ￥3500 In-stock
10 g   询价  
50 g   询价  

* Please select Quantity before adding items.

Berberine chloride hydrate 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Drug Repurposing Compound Library Plus
  • FDA-Approved Drug Library Plus
  • FDA-Approved Drug Library Mini
  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Cell Cycle/DNA Damage Compound Library
  • Immunology/Inflammation Compound Library
  • Metabolism/Protease Compound Library
  • NF-κB Signaling Compound Library
  • Stem Cell Signaling Compound Library
  • Natural Product Library
  • FDA-Approved Drug Library
  • Anti-Cancer Compound Library
  • Autophagy Compound Library
  • Anti-Aging Compound Library
  • Drug Repurposing Compound Library
  • Antioxidants Compound Library
  • Oxygen Sensing Compound Library
  • Antibacterial Compound Library
  • Ferroptosis Compound Library
  • Anti-COVID-19 Compound Library
  • NMPA-Approved Drug Library
  • Medicine Food Homology Compound Library
  • Pyroptosis Compound Library
  • Traditional Chinese Medicine Monomer Library
  • FDA Approved & Pharmacopeial Drug Library
  • Antibiotics Library
  • Alkaloids Library
  • Anti-Lung Cancer Compound Library
  • Mitochondria-Targeted Compound Library
  • Rare Diseases Drug Library

生物活性

Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) is an alkaloid that acts as an antibiotic. Berberine chloride hydrate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Antineoplastic properties[1].

IC50 & Target

ROS[1]
DNA topoisomerase[1]
体外研究
(In Vitro)

Berberine chloride hydrate ( Natural Yellow 18 chloride hydrate; 1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine chloride hydrate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine chloride hydrate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine chloride hydrate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 72 hours
Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay[1]

Cell Line: Colorectal carcinoma cell lines LoVo
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 24, 48, 72 hours
Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis[1]

Cell Line: LoVo cells
Concentration: 0, 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis[1]

Cell Line: LoVo cells
Concentration: 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.

体内研究
(In Vivo)

Berberine chloride hydrate (Natural Yellow 18 chloride hydrate; 10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine chloride hydrate at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
Dosage: 10, 30, or 50 mg/kg/day
Administration: Gastrointestinal gavage; for 10 consecutive days
Result: Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.

Clinical Trial

分子量

389.83

Formula

C20H20ClNO5
CAS 号

68030-18-2
中文名称

盐酸小檗碱水合物；盐酸黄连素水合物
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro: 

DMSO : ≥ 3.9 mg/mL (10.00 mM)

H2O : 1 mg/mL (2.57 mM; ultrasonic and warming and heat to 60°C)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5652 mL 12.8261 mL 25.6522 mL
5 mM 0.5130 mL 2.5652 mL 5.1304 mL
10 mM 0.2565 mL 1.2826 mL 2.5652 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Cai Y, et al. Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo. J Nat Med. 2014 Jan;68(1):53-62.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务