来自卡那霉素链霉菌的卡那霉素二硫酸盐

Description

Chemical structure: aminoglycosideKanamycin sulfate is a broad spectrum minoglycoside-antibiotic It is used as an additive in culture media for the isolation of group DStreptococcion Kanamycin Esculin Azide Agar (Mossel et al.) or for selection of transformed plant cells containing the neomycin phosphotransferase (for kanamycin resistance); on a kanamycin-medium. Kanamycin has been used for electrophysiological recordings of outer hair cells and inner hair cells. It is used to inhibit shoot regeneration from Siberian Elmleaf explants. It is recommended for use in cell culture applications at 100 mg/mL.

Preparation instructions

Kanamycin sulfate is soluble in water at 10 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.Kanamycin acid sulfate is prepared by solubilizing kanamycin sulfate in dilute sulfuric acid and drying. The sulfate content is approximately 25%.