潮霉素 B
基本信息
| 产品名称 | 潮霉素 B |
|---|---|
| 英文名称 | Hygromycin B |
| 别名 | 潮霉素 B 来源于吸水链霉菌 |
| 英文别名 | Hygromycin B from Streptomyces hygroscopicus |
| 规格或纯度 | 来自吸水链霉菌 |
| 运输条件 | 冰袋运输 |
| 生化机理 | 作用方式：本品通过诱导原核生物中m-RNA模板的误读，抑制蛋白质合成，具有抑制翻译的作用。 抗菌谱：潮霉素B对细菌、真菌和高等真核细胞有抑制作用。 |
一般描述
其他说明
仅用于生命科学研究。不可用于诊断。 Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.
制备说明
Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.
General Description
Formula: C 20 H 37 N 3 O 13 LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.) Molecular weight: Mr = 527.5 Working concentration: 50 – 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Application
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
Other Notes
For life science research only. Not for use in diagnostic procedures. Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.
相关属性
| CAS编号 | 31282-04-9 |
|---|---|
| 比旋光度 | 20° (C=1,H2O) |
| 熔点 | 160-180°C |
| 储存温度 | 2-8°C储存 |
| 分子量 | 527.52 |
| 分子式 | C20H37N3O13 |
| 品牌 | Jinpan |