次黄嘌呤

一般描述

次黄嘌呤(6-羟嘌呤)，一种嘌呤衍生物，是天然化合物。它是腺嘌呤的脱氨基形式，也是一磷酸腺苷(AMP)的分解产物。

应用

次黄嘌呤是一种营养添加剂，适用于细菌、寄生虫（ 恶性疟原虫 ）和动物细胞等多种细胞培养应用。 次黄嘌呤是杂交瘤技术所用选择培养基的组分。 次黄嘌呤已被用于： 作为HEPES (4-(2-羟乙基)-1-哌嗪乙烷磺酸) -buffered Roswell park memorial institute (RPMI)补充剂，培养 镰状疟原虫 菌株IT/FCR3 制备Dulbecco′改性′s培养基 (DMEM) + MXH，用于选择嵌合修饰痘苗病毒安卡拉株(MVA) 产生超氧阴离子 用于制备杂交瘤选择和维持培养基。

General Description

Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).

Application

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite ( Plasmodium falciparum ) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies. Hypoxanthine has been used: as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3 in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA) to generate superoxide anions to prepare hybridoma selection and maintenance media.