3-Methyladenine(Synonyms: 3-甲基腺嘌呤; 3-MA)

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3-Methyladenine (Synonyms: 3-甲基腺嘌呤; 3-MA) 纯度: 99.83%

3-Methyladenine (3-MA) 是 PI3K 的抑制剂。它通过抑制class III PI3K广泛作为自噬 (autophagy) 的抑制剂使用。

3-Methyladenine(Synonyms: 3-甲基腺嘌呤; 3-MA)

3-Methyladenine Chemical Structure

CAS No. : 5142-23-4

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3-Methyladenine 相关产品

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生物活性

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K[1].

IC50 & Target[1]

PtdIns3Kγ

60 μM (IC50, Cell Assay)

Vps34

25 μM (IC50, Cell Assay)

Autophagy

 

Mitophagy

 

Human Endogenous Metabolite

 

体外研究
(In Vitro)

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
3-Methyladenine induces cell death is independent of autophagy inhibition[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].
Note:
在体外细胞培养实验中,3-MA 常用的浓度为 0.5-10 mM,所以优先推荐直接称取本次实验所需粉末量,用培养基完全溶解,0.22 μm 过滤器过滤除菌之后使用。建议溶液现配现用,尽快用完。

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: HeLa cells
Concentration: 0 mM, 2.5 mM or 5 mM, 10 mM
Incubation Time: 0 hour, 24 hours and 48 hours
Result: Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

Cell Autophagy Assay[2]

Cell Line: HeLa cells
Concentration: 5 mM
Incubation Time: 24 hours
Result: Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

Western Blot Analysis[2]

Cell Line: HeLa cells
Concentration: 5 mM
Incubation Time: 0 hour, 12 hours, 24 hours and 48 hours
Result: Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.

体内研究
(In Vivo)

3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)[3]
Dosage: 1.5 mg/100 g (1000 μM)
Administration: Intraperitoneal injection
Result: Alleviated Sodium Taurocholate-Induced SAP.

分子量

149.15

Formula

C6H7N5

CAS 号

5142-23-4

中文名称

3-甲基腺嘌呤

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

H2O : 5 mg/mL (33.52 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 6.7047 mL 33.5233 mL 67.0466 mL
5 mM 1.3409 mL 6.7047 mL 13.4093 mL
10 mM 0.6705 mL 3.3523 mL 6.7047 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Miller S, et al. Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy. 2010 Aug;6(6):805-7.

    [2]. Hou H, et al. Inhibitors of phosphatidylinositol 3′-kinases promote mitotic cell death in HeLa cells. PLoS One. 2012;7(4):e35665.

    [3]. Wang X, et al. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats.Mediators Inflamm. 2016;2016:8369704.

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