3-Methyladenine (3-MA) 是 PI3K 的抑制剂。它通过抑制class III PI3K广泛作为自噬 (autophagy) 的抑制剂使用。
3-Methyladenine Chemical Structure
CAS No. : 5142-23-4
规格
价格
是否有货
数量
Free Sample (0.1-0.5 mg)
Apply now
50 mg
¥550
In-stock
100 mg
¥800
In-stock
200 mg
¥1400
In-stock
500 mg
¥2800
In-stock
1 g
询价
5 g
询价
* Please select Quantity before adding items.
3-Methyladenine 相关产品
•相关化合物库:
Natural Product Library Plus
Bioactive Compound Library Plus
生物活性
3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K[1].
IC50 & Target[1]
PtdIns3Kγ
60 μM (IC50, Cell Assay)
Vps34
25 μM (IC50, Cell Assay)
Autophagy
Mitophagy
Human Endogenous Metabolite
体外研究 (In Vitro)
3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2]. 3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2]. 3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2]. 3-Methyladenine induces cell death is independent of autophagy inhibition[2]. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2]. Note: 在体外细胞培养实验中,3-MA 常用的浓度为 0.5-10 mM,所以优先推荐直接称取本次实验所需粉末量,用培养基完全溶解,0.22 μm 过滤器过滤除菌之后使用。建议溶液现配现用,尽快用完。
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[2]
Cell Line:
HeLa cells
Concentration:
0 mM, 2.5 mM or 5 mM, 10 mM
Incubation Time:
0 hour, 24 hours and 48 hours
Result:
Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.
Cell Autophagy Assay[2]
Cell Line:
HeLa cells
Concentration:
5 mM
Incubation Time:
24 hours
Result:
Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.
Western Blot Analysis[2]
Cell Line:
HeLa cells
Concentration:
5 mM
Incubation Time:
0 hour, 12 hours, 24 hours and 48 hours
Result:
Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.
体内研究 (In Vivo)
3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours[3]. 3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3]. 3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)[3]
Dosage:
1.5 mg/100 g (1000 μM)
Administration:
Intraperitoneal injection
Result:
Alleviated Sodium Taurocholate-Induced SAP.
分子量
149.15
Formula
C6H7N5
CAS 号
5142-23-4
中文名称
3-甲基腺嘌呤
运输条件
Room temperature in continental US; may vary elsewhere.
Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic
*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
[1]. Miller S, et al. Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy. 2010 Aug;6(6):805-7.
[2]. Hou H, et al. Inhibitors of phosphatidylinositol 3′-kinases promote mitotic cell death in HeLa cells. PLoS One. 2012;7(4):e35665.
[3]. Wang X, et al. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats.Mediators Inflamm. 2016;2016:8369704.