DMU-212

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DMU-212  纯度: 99.91%

DMU-212 是一种白藜芦醇 (HY-16561) 甲基化衍生物，具有抗分裂、抗增殖、抗氧化和促进细胞凋亡的活性。DMU-212 通过诱导凋亡 (apoptosis) 和激活 ERK1/2 蛋白导致有丝分裂停止。DMU-212 具有口服活性。

DMU-212

DMU-212 Chemical Structure

CAS No. : 134029-62-2

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1100 In-stock
10 mg ￥1000 In-stock
25 mg ￥2000 In-stock
50 mg ￥3500 In-stock
100 mg   询价  
200 mg   询价  

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生物活性

DMU-212 is a methylated derivative of Resveratrol (HY-16561), with antimitotic, anti-proliferative, antioxidant and apoptosis promoting activities. DMU-212 induces mitotic arrest via induction of apoptosis and activation of ERK1/2 protein. DMU-212 has orally active[1][2].

体外研究
(In Vitro)

DMU-212 (0.3125-40 μM) inhibits growth of A375, MeWo, Bro and M5 human melanoma cells[1].
DMU-212 (30-50 μM; 24 hours) induces upregulation of cell cycle inhibitors, apoptosis and ERK activation in A375 cells[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: A375 cells, MeWo cells, M5 cells, Bro cells
Concentration: 0.3125 μM, 0,625 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 96 hours
Result: Inhibited the cellular proliferation of human melanoma cells at submicromolar or micromolar concentrations (IC50=0.5 μM for A375 and Bro and IC50= 1.25 μM for MeWo and M5 cells).

Cell Cycle Analysis[1]

Cell Line: A375 cells
Concentration: 20 μM, 30 μM, 50 μM
Incubation Time: 24 hours
Result: Caused a marked increase in the levels of p21, p53 and cyclin B1 proteins with a concomitant decrease in the levels of cyclin A2.

Western Blot Analysis[1]

Cell Line: A375 cells
Concentration: 20 μM, 30 μM, 50 μM
Incubation Time: 24 hours
Result: Significant upregulated Bax, caspase 3 and caspase 9 protein levels, while decreased the levels of the anti-apoptotic protein Bcl-2.

Apoptosis Analysis[1]

Cell Line: A375 cells
Concentration: 10 μM, 20 μM
Incubation Time: 24 hours, 36 hours
Result: Induced apoptosis.

体内研究
(In Vivo)

DMU-212 (50 mg/kg; i.g.; three times a week; for 14 days) inhibits tumor growth in xenograft model of human ovarian cancer[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6-weeks-old SCID female mice (20-24 g), with ovarian cancer xenografts[2]
Dosage: 50 mg/kg
Administration: Oral gavage, three times a week, for 14 days
Result: Lowered tumor burden.

分子量

300.35

Formula

C18H20O4
CAS 号

134029-62-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 20 mg/mL (66.59 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3294 mL 16.6472 mL 33.2945 mL
5 mM 0.6659 mL 3.3294 mL 6.6589 mL
10 mM 0.3329 mL 1.6647 mL 3.3294 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2 mg/mL (6.66 mM); Clear solution

    此方案可获得 ≥ 2 mg/mL (6.66 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Vasilis Pericles Androutsopoulos, et al. Activation of ERK1/2 is required for the antimitotic activity of the resveratrol analogue 3,4,5,4′-tetramethoxystilbene (DMU-212) in human melanoma cells. Exp Dermatol. 2015 Aug;24(8):632-4.

    [2]. Hanna Piotrowska, et al. DMU-212 inhibits tumor growth in xenograft model of human ovarian cancer. Biomed Pharmacother. 2014 May;68(4):397-400.

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