CCT007093

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

CCT007093  纯度: ≥98.0%

CCT007093 是有效的蛋白磷酸酶 1D (PPM1D Wip1) 的抑制剂。Wip1 的抑制可激活mTORC1 通路，增强肝切除手术后肝细胞的增殖。

CCT007093

CCT007093 Chemical Structure

CAS No. : 176957-55-4

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥830 In-stock
10 mg ￥750 In-stock
50 mg ￥2500 In-stock
100 mg ￥4500 In-stock
500 mg ￥12500 询价
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

CCT007093 相关产品

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生物活性

CCT007093 is an effective protein phosphatase 1D (PPM1D Wip1) inhibitor. Wip1 inhibition can activate the mTORC1 pathway and enhance hepatocyte proliferation after hepatectomy[1][2].

体外研究
(In Vitro)

CCT007093 (25 or 50 µM, 8 h) significantly increases the phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159, and the phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) are also up-regulated in transfected HEK293T cell line[1].
CCT007093 shows specificity for MCF-7 cells, reducing viability by 40% after 2 days with no observable effect on the growth of HeLa cells[2].
CCT007093 induces P38 phosphorylation at 4 h post exposure in MCF-7 cells (sensitive to PPM1D inhibition), and does not induce P38 phosphorylation in HeLa cells (relatively resistant to PPM1D inhibition)[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HEK293T cell line.
Concentration: 25 or 50 µM.
Incubation Time: 8 h.
Result: The phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159 were all significantly increased[1].
The phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) were also up-regulated[1].

体内研究
(In Vivo)

CCT007093 (6.4 mg/kg) enhances liver regeneration and increases the survival rate of mice following major hepatectomy[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wild-type mice[1].
Dosage: 3.2 and 6.4 mg/kg.
Administration: Injected intraperitoneally 4 times (mice were sacrificed at 36 h post-PH).
Result: Significantly improved survival in mice following major hepatectomy (80% hepatectomy).
The level of PCNA was also significantly increased in the liver of CCT007093-treated mice at 36h, 48h and 72h post-PH.

分子量

272.39

Formula

C15H12OS2
CAS 号

176957-55-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMF : 3.33 mg/mL (12.23 mM; Need ultrasonic)

DMSO : ≥ 2.8 mg/mL (10.28 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.6712 mL 18.3560 mL 36.7121 mL
5 mM 0.7342 mL 3.6712 mL 7.3424 mL
10 mM 0.3671 mL 1.8356 mL 3.6712 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Lingling Zhang, et al. Inhibition of wild-type p53-induced phosphatase 1 promotes liver regeneration in mice by direct activation of mammalian target of rapamycin. Hepatology. 2015 Jun;61(6):2030-41.

    [2]. S Rayter, et al. A chemical inhibitor of PPM1D that selectively kills cells overexpressing PPM1D. Oncogene. 2008 Feb 14;27(8):1036-44.

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