SRT 1460 | 赛默飞Alfa aesar官网

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SRT 1460 纯度: 98.92%

SRT 1460 是一种有效的 Sirtuin-1 (SIRT1) 激动剂，其 EC_1.5 值为 2.9 μM。与对 SIRT2 和 SIRT3 (EC_1.5>300 μM) 相比，SRT 1460 对 SIRT1 有很好的选择性，并且比白藜芦醇和相近 sirtuin 同系物更为有效。

SRT 1460 Chemical Structure

CAS No. : 925432-73-1

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥1120	In-stock
5 mg	￥1000	In-stock
10 mg	￥1600	In-stock
25 mg	￥2300	In-stock
50 mg	￥3500	In-stock
100 mg	￥5300	In-stock
200 mg		询价
500 mg		询价

* Please select Quantity before adding items.

SRT 1460 相关产品

•相关化合物库:

Bioactive Compound Library Plus
Cell Cycle/DNA Damage Compound Library
Epigenetics Compound Library
Histone Modification Research Compound Library
Anti-Cancer Compound Library
Anti-Aging Compound Library
Antioxidants Compound Library
Oxygen Sensing Compound Library
Glutamine Metabolism Compound Library
Anti-Pancreatic Cancer Compound Library

生物活性

SRT 1460, a potent Sirtuin-1 (SIRT1) activator with an EC_1.5 value of 2.9 μM, shows good selectivity for activation of SIRT1 versus SIRT2 and SIRT3 (EC1.5>300 μM), and is more potent than Resveratrol and the closest sirtuin homologues^[1].

IC₅₀ & Target^[1]

SIRT1

2.9 μM (EC1.5)

体外研究
(In Vitro)

SRT 1460 (2-6 μM; 72 hours) inhibits cell viability in a dose-dependent manner, with all pancreatic cancer cells being more sensitive than the control HPDE cell. The IC₅₀s of those cells are: Patu8988t, 1.62±0.13 μM; SU86.86, 2.31±0.23 μM; Panc-1, 0.66 ±0.02 μM; HPDE, 2.39±0.29 μM^[2].
SRT 1460 (5 μM; 16 hours) increases expression of the autophagy marker LC3-II^[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Patu8988t (pancreatic cancer cells), Panc-1 (pancreatic cancer cells), SU86.86 (pancreatic cancer cells), HPDE cells
Concentration:	2 μM, 4 μM, 6 μM
Incubation Time:	72 hours
Result:	Inhibited cell viability in a dose-dependent manner, with all pancreatic cancer cells being more sensitive than the control HPDE cell. The IC₅₀s of those cells were: Patu8988t, 1.62±0.13 μM; SU86.86, 2.31±0.23 μM; Panc-1, 0.66 ±0.02 μM; HPDE, 2.39±0.29 μM.

Western Blot Analysis^[2]

Cell Line:	Patu8988t cells
Concentration:	5 μM
Incubation Time:	16 hours
Result:	SRT1460 increased expression of the autophagy marker LC3-II.

分子量

507.60

Formula

C₂₆H₂₉N₅O₄S

CAS 号

925432-73-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 10 mg/mL (19.70 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9701 mL	9.8503 mL	19.7006 mL
5 mM	0.3940 mL	1.9701 mL	3.9401 mL
10 mM	0.1970 mL	0.9850 mL	1.9701 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 1.25 mg/mL (2.46 mM); Clear solution

此方案可获得 ≥ 1.25 mg/mL (2.46 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil

Solubility: ≥ 1.25 mg/mL (2.46 mM); Clear solution

此方案可获得 ≥ 1.25 mg/mL (2.46 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在上海金畔生物科技有限公司网站选购。

参考文献

[1]. Milne JC, et al. Small molecule activators of SIRT1 as therapeutics for the treatment of type 2 diabetes. Nature. 2007 Nov 29; 450(7170): 712–716.

[2]. Chini CC, et al. SIRT1-Activating Compounds (STAC) Negatively Regulate Pancreatic Cancer Cell Growth and Viability Through a SIRT1 Lysosomal-Dependent Pathway. Clin Cancer Res. 2016 May 15;22(10):2496-507.

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