STAT5-IN-2

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STAT5-IN-2  纯度: 99.01%

STAT5-IN-2 是 STAT5 的抑制剂,在 K562 和 KU812 细胞中的 EC50 分别为 9 μM和 5 μM,在文献 1 的名称是 17f。STAT5-IN-2 具有高效的抗白血病活性。

STAT5-IN-2

STAT5-IN-2 Chemical Structure

CAS No. : 2111834-61-6

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥4400 In-stock
5 mg ¥4000 In-stock
10 mg ¥6800 In-stock
50 mg ¥19500 In-stock
100 mg ¥28000 In-stock
200 mg   询价  
500 mg   询价  

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STAT5-IN-2 相关产品

相关化合物库:

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生物活性

STAT5-IN-2 is a STAT5 inhibitor, extracted from reference 1, example 17f. STAT5-IN-2 has potent antileukemic effect[1].

IC50 & Target[1]

STAT5

9 μM (EC50, in K562 cells)

STAT5

5 μM (EC50, in KU812 cells )

体外研究
(In Vitro)

STAT5-IN-2 has EC50 values of 9 μM and 5 μM in K562 and KU812 cells, respectively[1].
STAT5-IN-2 (100 nM-50 µM; 48 hours) has EC50 values of 2.6 µM and 3.5 µM in KG1a and MV-4-11 cells, respectively[1].
STAT5-IN-2 (10 µM; 48 hours) induces apoptosis in all cell lines (K562, KU812, KG1a and MV-4-11 cells)[1].
STAT5-IN-2 (10 µM; 24 hours) blocks phosphorylation of STAT5[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: KG1a and MV-4-11 cell lines
Concentration: 100 nM, 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 48 hours
Result: Inhibited cell growth in KG1a and MV-4-11 cell lines

Apoptosis Analysis[1]

Cell Line: K562, KU812, KG1a and MV-4-11 cells
Concentration: 10 µM
Incubation Time: 48 hours
Result: Significantly increased the number of apoptotic cells.

Western Blot Analysis[1]

Cell Line: KG1a, MV-4-11 and KU812 cells
Concentration: 10 µM
Incubation Time: 24 hours
Result: Inhibited phosphorylation of STAT5 compared to no influence on phosphorylation level of STAT3, Akt and Erk1/2.

分子量

397.51

Formula

C26H27N3O

CAS 号

2111834-61-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (251.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5157 mL 12.5783 mL 25.1566 mL
5 mM 0.5031 mL 2.5157 mL 5.0313 mL
10 mM 0.2516 mL 1.2578 mL 2.5157 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.29 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.29 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Ludovic Juen, et al. New Inhibitor Targeting Signal Transducer and Activator of Transcription 5 (STAT5) Signaling in Myeloid Leukemias. J Med Chem. 2017 Jul 27;60(14):6119-6136.

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