Silymarin(Synonyms: 水飞蓟素)

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Silymarin (Synonyms: 水飞蓟素) 纯度: ≥80.0%

Silymarin 是水飞蓟 (Silybum marianum) 的提取物。Silymarin 是一种有效的 SARS-CoV-2 主蛋白酶 (Mpro) 抑制剂。Silymarin 可以显着降低肿瘤细胞的增殖，血管生成以及胰岛素抵抗。Silymarin 对于肝细胞癌 (HCC) 具有化学预防作用。Silymarin 具有用于 COVID-19 研究的潜力。

Silymarin(Synonyms: 水飞蓟素)

Silymarin Chemical Structure

CAS No. : 65666-07-1

规格 价格 是否有货 数量
250 mg ￥500 In-stock
500 mg ￥750 In-stock
1 g   询价  
5 g   询价  

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Silymarin 相关产品

•相关化合物库:

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生物活性

Silymarin is an extract of the milk thistle (Silybum marianum). Silymarin is an effective SARS-CoV-2 main protease (Mpro) inhibitor. Silymarin can significantly reduce tumor cell proliferation, angiogenesis as well as insulin resistance. Silymarin has the chemopreventive effect on hepatocellular carcinoma (HCC). Silymarin has the potential for COVID-19 research[1][2][4].

体外研究
(In Vitro)

Silymarin (0-120 µg/ml; 24 hours) inhibits AGS cell viability, the viability of AGS cells is 77.9% in the presence of 20 µg/ml silymarin, 71.5% at 40 µg/ml, 59.8% at 60 µg/ml, 44.5% at 80 µg/ml, 35.3% at 100 µg/ml and 33.9% at 120 µg/ml[1].
Silymarin (40-80 µg/ml; 24 hours) inhibits the migration of the AGS cells in a concentration-dependent manner. It inhibit migration of AGS cells 59.4% at 40 µg/ml and 21.7% at 80 µg/ml,respectively[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: AGS cells
Concentration: 20 µg/ml, 40 µg/ml, 80 µg/ml, 100 µg/ml and 120 µg/ml
Incubation Time: 24 hours
Result: Indicated a significant concentration-dependent inhibitory effect on AGS cells starting at 20 µg/ml.

体内研究
(In Vivo)

Silymarin (oral gavage; 10, 20, 50, 100, and 200 mg/kg) decreases the immobility time in a dose-dependent manner in forced swimming test (FST). It also lowers the immobility measure dose-dependently in tail suspension test (TST). Additionally, 50% of maximum response (ED50) of Silymarin is around 10 mg/kg. The dose 100 mg/kg proved the most effective dose in both the tests[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

CAS 号

65666-07-1
中文名称

水飞蓟素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 120 mg/mL (Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 3 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 3 mg/mL (Infinity mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 30.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Féher J, et al. Silymarin in the prevention and treatment of liver diseases and primary liver cancer. Curr Pharm Biotechnol. 2012 Jan;13(1):210-7.

    [2]. Sung-Hyun Kim, et al.Silymarin induces inhibition of growth and apoptosis through modulation of the MAPK signaling pathway in AGS human gastric cancer cells. Oncol Rep. 2019 Nov;42(5):1904-1914.

    [3]. Mina Khoshnoodi, et al. Possible involvement of nitric oxide in antidepressant-like effect of silymarin in male mice. Pharm Biol. 2015 May;53(5):739-45.

    [4]. Anna Maria Sardanelli, et al. SARS-CoV-2 Main Protease Active Site Ligands in the Human Metabolome. Molecules 2021, 26(5), 1409.

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