Okadaic acid ammonium salt(Synonyms: 冈田酸铵盐)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Okadaic acid ammonium salt (Synonyms: 冈田酸铵盐)

Okadaic acid ammonium salt 是一种海洋毒素,是蛋白质磷酸酶 (PP) 的抑制剂,包括 PP1 (IC50=15-50 nM)、PP2A (IC50=0.1-0.3 nM)、PP3 (IC50=3.7-4 nM)、PP4 (IC50=0.1 nM) 和 PP5 (IC50=3.5 nM),其中对 PP2A 的亲和力最显著,但不抑制 PP2C。Okadaic acid ammonium salt 通过抑制 PP 增加了多种蛋白的磷酸化,并具有肿瘤启动子的作用。Okadaic acid ammonium salt 诱导 tau 蛋白磷酸化。

Okadaic acid ammonium salt(Synonyms: 冈田酸铵盐)

Okadaic acid ammonium salt Chemical Structure

CAS No. : 175522-42-6

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Okadaic acid ammonium salt 的其他形式现货产品:

Okadaic acid

生物活性

Okadaic acid ammonium salt, a marine toxin, is an inhibitor of protein phosphatases (PP). Okadaic acid ammonium salt has a significantly higher affinity for PP2A (IC50=0.1-0.3 nM), and inhibits PP1 (IC50=15-50 nM), PP3 (IC50=3.7-4 nM), PP4 (IC50=0.1 nM), PP5 (IC50=3.5 nM), but does not inhibit PP2C. Okadaic acid ammonium salt increases of phosphorylation of a number of proteins by inhibiting PP, and acts as a tumor promoter. Okadaic acid ammonium salt induces tau phosphorylation[1][2].

IC50 & Target[1]

PP1

15-50 nM (IC50)

PP2A

0.1-0.3 nM (IC50)

PP3

3.7-4 nM (IC50)

PP4

0.1 nM (IC50)

PP5

3.5 nM (IC50)

PP2B

~4000 nM (IC50)

PP7

>1000 nM (IC50)

体外研究
(In Vitro)

Okadaic acid ammonium salt (0-100 nM; 24 h or 48 h) inhibits the proliferation of AGS, MNK-45, Caco 2 cells[3].
Okadaic acid (10 nM, 8 hours) ammonium salt increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons[4].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[3]

Cell Line: AGS, MNK-45 and Caco 2 cell lines
Concentration: 0-100 nM
Incubation Time: 24 h or 48 h
Result: Inhibited the proliferation of AGS, MNK-45, Caco 2 cells.

体内研究
(In Vivo)

Okadaic acid ammonium salt (100 μM; injected unilaterally to the lateral amygdala) induces Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection[5].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female wild-type C57BL/6 mice (6 to 8 months)[5]
Dosage: 100 μM
Administration: Injected unilaterally to the lateral amygdala
Result: Induced Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection.

分子量

822.03

Formula

C44H71NO13

CAS 号

175522-42-6

中文名称

冈田酸铵盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Kleppe R, et al. Cell Death Inducing Microbial Protein Phosphatase Inhibitors–Mechanisms of Action. Mar Drugs. 2015 Oct 22;13(10):6505-20.

    [2]. Valdiglesias V, et al. Okadaic acid: more than a diarrheic toxin. Mar Drugs. 2013 Oct 31;11(11):4328-49.

    [3]. del Campo M, et al. Okadaic acid toxin at sublethal dose produced cell proliferation in gastric and colon epithelial cell lines. Mar Drugs. 2013;11(12):4751-4760.

    [4]. Cho MH, et al. Increased phosphorylation of dynamin-related protein 1 and mitochondrial fission in okadaic acid-treated neurons. Brain Res. 2012 May 15;1454:100-10.

    [5]. Baker S, et al. A local insult of okadaic acid in wild-type mice induces tau phosphorylation and protein aggregation in anatomically distinct brain regions. Acta Neuropathol Commun. 2016;4:32.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务