RAMB4

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

RAMB4  纯度: 98.43%

RAMB4 是通过抑制 20S 蛋白酶体催化活性物质上游泛素介导的蛋白质降解而产生的泛素-蛋白酶体系统 (UPS) 应激源。RAMB4 在不影响 20S 蛋白酶体催化活性的情况下触发泛素蛋白酶体系统 (UPS) 应激反应。

RAMB4

RAMB4 Chemical Structure

CAS No. : 145888-79-5

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥990 In-stock
10 mg ￥900 In-stock
50 mg ￥2500 In-stock
100 mg ￥4200 In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

RAMB4 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Metabolism/Protease Compound Library
  • Anti-Cancer Compound Library
  • Ubiquitination Compound Library
  • Anti-Blood Cancer Compound Library

生物活性

RAMB4 is a ubiquitin-proteasome system (UPS)-stressor. RAMB4 inhibits ubiquitin-mediated protein degradation upstream of the 20S proteasomal catalytic activites. RAMB4 triggers a ubiquitin-proteasome-system (UPS)-stress response without affecting 20S proteasome catalytic activities. Anticancer activity[1].

体外研究
(In Vitro)

RAMB4 (0-30 µM; 48 hours) treatment produces a dose dependent reduction in the viability of HPV16-positive SiHa and Caski cells and HPV-39-positive ME180 cervical cancer cell lines respectively[1].
RAMB4 reduces the cell viability of exponentially growing HeLa cervical cancer cells in a dose-dependent fashion with an IC50 value of 2 µM[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Keratinocytes, SiHa, CaSki and ME180 cells
Concentration: 5, 10, 15, 20, 25, 30 µM
Incubation Time: 48 hours
Result: Produced a dose dependent reduction in the viability of HPV16-positive SiHa and Caski cells and HPV-39-positive ME180 cervical cancer cell lines respectively with minimal effects on the viability of primary human keratinocytes and with IC50 similar to the obtained with HeLa cells.

分子量

413.12

Formula

C19H13Cl4NO
CAS 号

145888-79-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 5 mg/mL (12.10 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4206 mL 12.1030 mL 24.2060 mL
5 mM 0.4841 mL 2.4206 mL 4.8412 mL
10 mM 0.2421 mL 1.2103 mL 2.4206 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Anchoori RK, et al. Stressing the ubiquitin-proteasome system without 20S proteolytic inhibition selectively kills cervical cancer cells.PLoS One. 2011;6(8):e23888.

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