FM19G11

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

FM19G11 

FM19G11 是一种 hypoxia-inducible factor-1-alpha (HIF-1α) 抑制剂,在 HeLa 细胞中抑制缺氧诱导的荧光素酶活性,其 IC50 值为 80 nM。当 HIF-1α 途径在常氧条件下失活时,FM19G11 会调节其他信号途径,包括 mTORPI3K/Akt/eNOS

FM19G11

FM19G11 Chemical Structure

CAS No. : 329932-55-0

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生物活性

FM19G11 is a hypoxia-inducible factor-1-alpha (HIF-1α) inhibitor, and it inhibits hypoxia-induced luciferase activity with an IC50 of 80 nM in HeLa cells. FM19G11 modulates other signaling pathways, including mTOR and PI3K/Akt/eNOS, when the HIF-1α pathway is inactivated under normoxic conditions[1][2].

IC50 & Target

HIF-1α[1]

体外研究
(In Vitro)

FM19G11 (30-300 nM) inhibits HIFα proteins in the HeLa cell lines[1].
FM19G11 (500 nM) promotes oligodendrocyte differentiation under hypoxia[1].
FM19G11 (300 nM; 3 days) suppresses the mRNA levels of O6-methylguanine DNA-methyltransferase (MGMT) significantly in hypoxic GBM‐XD, hypoxic T98G, and normoxic T98G cells[2].
M19G11 (300 nM; 3 days) significantly enhances the pro‐apoptotic effect of temozolomide (TMZ), although FM19G11 does not induce apoptosis by itself[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: GBM‐XD and T98G cells
Concentration: 300 nM
Incubation Time: 3 days
Result: Had no cytotoxicity by itself.
Enhanced the cytotoxicity of TMZ in hypoxic GBM-XD cells, hypoxic T98G cells, and normoxic T98G cells.

Western Blot Analysis[2]

Cell Line: GBM‐XD and T98G cells
Concentration: 300 nM
Incubation Time: 3 days
Result: Suppressed MGMT expression significantly in both cell lines in hypoxic culture.
Downregulated MGMT expression substantially inT98G cells in normoxic culture.

体内研究
(In Vivo)

FM19G11 ( intramedullary injection; 1-8 weeks) improves locomotion in severe spinal cord injury (SCI)[3].
FM19G11 ( intramedullary injection; 8 weeks) induces the expression of GAP43, an axon growth marker, and RIP, a marker for myelinated oligodendrocytes at the injury[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

463.40

Formula

C23H17N3O8

CAS 号

329932-55-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Victoria MM, et, al. FM19G11, a new hypoxia-inducible factor (HIF) modulator, affects stem cell differentiation status. J Biol Chem. 2010 Jan 8; 285(2): 1333-42.

    [2]. You CG, et, al. FM19G11 inhibits O 6 -methylguanine DNA-methyltransferase expression under both hypoxic and normoxic conditions. Cancer Med. 2018 May 15; 7(7): 3292-3300.

    [3]. Ana AA, et, al. FM19G11 and Ependymal Progenitor/Stem Cell Combinatory Treatment Enhances Neuronal Preservation and Oligodendrogenesis after Severe Spinal Cord Injury. Int J Mol Sci. 2018 Jan 9; 19(1): 200.

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