Streptozocin is a potent DNA-methylating antibiotic. Streptozotocin causes methylation of liver and kidney and pancreatic DNA, but no methylation in brain DNA.

DNA alkylator^[1]

Streptozocin (STZ) shows higher cytotoxic effect in vitro on hematological cell lines compared to Alloxan (ALX). ALX appeares not to be toxic for the studied cell lines with estimated IC₅₀ values of 2809, 3679 or over 4000 μg/mL for HL60, K562 and C1498 cells, respectively. Streptozocin is more toxic, especially for the human myeloid leukemia cell line, HL60. The IC₅₀ values of Streptozocin are 11.7, 904 and 1024 μg/mL for HL60, K562 and C1498 cells, respectively. Results also show that the murine leukemic cells are more resistant to Streptozocin and ALX cytotoxicity than human leukemic cells^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Streptozocin (STZ)-injected mice show tendency to have lower body weight than that observed in animals injected with ALX. Streptozocin -injected mice have significantly fewer splenocytes (22.2±3.2×10⁶; n=10) compared to mice injected with ALX (60.7±4.3×10⁶; n=15; p=0.01)^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

265.22

C₈H₁₅N₃O₇

18883-66-4

链脲霉素；链佐星；链脲佐菌素

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

DMSO : 125 mg/mL (471.31 mM; Need ultrasonic)

H₂O : 113.3 mg/mL (427.19 mM; Need ultrasonic and warming)

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 130 mg/mL (490.16 mM); Clear solution; Need ultrasonic
• 2.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 2.08 mg/mL (7.84 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (7.84 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 3.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.08 mg/mL (7.84 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (7.84 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 4.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.08 mg/mL (7.84 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (7.84 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Bennett RA, et al. Alkylation of DNA in rat tissues following administration of streptozotocin. Cancer Res. 1981 Jul;41(7):2786-90

  [2]. Diab RA, et al. Immunotoxicological effects of streptozotocin and alloxan: in vitro and in vivo studies. Immunol Lett. 2015 Feb;163(2):193-8

  [3]. Acer S, et al. Oxidative stress of crystalline lens in rat menopausal model. Arq Bras Oftalmol. 2016 Jul-Aug;79(4):222-5.

Human and murine cell lines are cultured in triplicate in 96-well plates at a density of 2×10⁴ cells/well in the absence (untreated control) or presence of various concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 h at 37°C under a humidified atmosphere containing 5% CO₂. Cells incubated in complete media including dH₂O in a final concentration of 0.1% served as control for solvent toxicity and cells incubated in complete medium are used as a control for the experiments. The effects of the tested drugs on tumor cell growth or viability are determined employing the MTT assay in accordance with the manufacturer’s instructions. The IC₅₀values (drug concentration that induces 50% inhibition of the cell growth) are calculated using the GraphPad Prism 4 program^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Mice^[2]
Male C57BL/6 mice (10-16 weeks) are used.The age group distribution in the mouse group treated with Streptozocin and ALX as well as controls is as follows: Streptozocin xenograft (n=7, median age 14 weeks), ALX xenograft (n=11, median age 15 weeks), Streptozocin non-transplanted (n=7, median age 14 weeks), ALX non-transplanted (n=15, median age 15 weeks).Male C57BL/6 mice are under inhalation anesthesia injected via the penile vein with ALX (75 mg/mL) or Streptozocin (180 mg/kg). Control group contain male C57BL/6 mice. Blood glucose levels and body weight are measured before injection, after 6 h, then daily after drug injection.
Rats^[3]
Thirty rats underwent oophorectomy to induce menopausal status. Rats receive intraperitoneal administration of Streptozocin (50 mg/kg) to induce diabetes mellitus (DM) 1 week after the oophorectomy. Blood glucose level is checked 3 days after Streptozocin administration, and values >250 mg/dL are considered as positive for DM.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Bennett RA, et al. Alkylation of DNA in rat tissues following administration of streptozotocin. Cancer Res. 1981 Jul;41(7):2786-90

  [2]. Diab RA, et al. Immunotoxicological effects of streptozotocin and alloxan: in vitro and in vivo studies. Immunol Lett. 2015 Feb;163(2):193-8

  [3]. Acer S, et al. Oxidative stress of crystalline lens in rat menopausal model. Arq Bras Oftalmol. 2016 Jul-Aug;79(4):222-5.