JG-98

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

JG-98  纯度: 99.75%

JG-98 是一种热休克蛋白 70 (Hsp70) 变构抑制剂，可与 Hsp70 上的保守位点紧密结合，打断 Hsp70-Bag3 相互作用。JG-98 影响癌细胞和肿瘤相关巨噬细胞，显示抗癌活性。

JG-98

JG-98 Chemical Structure

CAS No. : 1456551-16-8

规格 价格 是否有货 数量
5 mg ￥3500 In-stock
10 mg ￥5500 In-stock
50 mg 询价

* Please select Quantity before adding items.

JG-98 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Cell Cycle/DNA Damage Compound Library
  • Metabolism/Protease Compound Library
  • Anti-Cancer Compound Library
  • Anti-Aging Compound Library
  • Cytoskeleton Compound Library

生物活性

JG-98, an allosteric heat shock protein 70 (Hsp70) inhibitor, which binds tightly to a conserved site on Hsp70 and disrupts the Hsp70-Bag3 interaction. JG-98 shows anti-cancer activities affecting both cancer cells and tumor-associated macrophages[1][2][3].

IC50 & Target

Heat shock protein 70[1]
体外研究
(In Vitro)

JG-98 (30 nM-30 μM; 72 hours) has antiproliferative activity across a range of cell lines with the EC50s between ~0.3 and 4 μM[2].
JG-98 (10 μM; 48 hours) activates apoptotic mediators (cleavage of caspase-3 and PARP) in MDA-MB-231 cells[1].
JG-98 destabilizes FoxM1 and relieves suppression of downstream effectors, including p21 and p27[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: MCF-7, MDA-MB-231, A375, MeWo, HeLa, HT-29, SKOV3, Jurkat, mouse embryonic fibroblasts (MEF), MM1.R, INA6, RPMI-8226, JJN-3, U266, NCI-H929, L363, MM1.S, KMS11, LP-1, AMO-1, OPM1, OPM2 cells
Concentration: 30 nM-30 μM
Incubation Time: 72 hours
Result: Active against all of the lines tested, and the EC50s were variable (between ~0.3 μM and 4 μM). Normal MEFs and OPM1 and OPM2 were relatively less sensitive.

Western Blot Analysis[1]

Cell Line: MDA-MB-231 cells
Concentration: 10 μM
Incubation Time: 48 hours
Result: Incudes apoptotic mediators cleavage of caspase 3 and PARP.

体内研究
(In Vivo)

JG-98 (3 mg/kg; i.p.; on days 0, 2, and 4) suppresses tumor growth in xenograft models bearing MCF7 and HeLa cells[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6-week-old NCR mice (xenografts of MCF7 and HeLa cells)[2]
Dosage: 3 mg/kg
Administration: Intraperitoneal injection; on days 0, 2, and 4
Result: Limited tumor growth, but somewhat less effectively.

分子量

534.54

Formula

C24H21Cl2N3OS3
CAS 号

1456551-16-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro: 

DMSO : 5 mg/mL (9.35 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8708 mL 9.3538 mL 18.7077 mL
5 mM 0.3742 mL 1.8708 mL 3.7415 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Li X, et al. Analogs of the Allosteric Heat Shock Protein 70 (Hsp70) Inhibitor, MKT-077, as Anti-Cancer Agents. ACS Med Chem Lett. 2013 Nov 14;4(11).

    [2]. Li X, et al. Validation of the Hsp70-Bag3 protein-protein interaction as a potential therapeutic target in cancer. Mol Cancer Ther. 2015 Mar;14(3):642-8.

    [3]. Yaglom JA, et al. Cancer cell responses to Hsp70 inhibitor JG-98: Comparison with Hsp90 inhibitors and findingsynergistic drug combinations. Sci Rep. 2018 Feb 14;8(1):3010.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务