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DDR Inhibitor 纯度: 99.43%

DDR Inhibitor 是一种高效的盘状结构域受体 (discoidin domain receptor) 抑制剂，对 DDR2 的 IC₅₀ 值为 3.3 nM，在浓度为 1.5 nM 时，对 DDR1 有 53% 的抑制作用。

DDR Inhibitor Chemical Structure

CAS No. : 1644069-80-6

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥7040	In-stock
5 mg	￥6400	In-stock
10 mg	￥10240	In-stock
50 mg		询价
100 mg		询价

* Please select Quantity before adding items.

DDR Inhibitor 相关产品

•相关化合物库:

Bioactive Compound Library Plus

生物活性

DDR Inhibitor is a potent discoidin domain receptor (DDR) inhibitor, with an IC₅₀ of 3.3 nM for DDR2, and shows 53% inhibition on DDR1 at 1.5 nM.

IC₅₀ & Target

IC50: 3.3 nM (DDR2)^[1]
IC53: 1.5 nM (DDR1)^[1]

体外研究
(In Vitro)

DDR Inhibitor (Example 6) is a potent DDR inhibitor, with an IC₅₀ of 3.3 nM for DDR2, and shows 53% inhibition on DDR1 at 1.5 nM^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

417.44

Formula

C₂₃H₂₀FN₅O₂

CAS 号

1644069-80-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 65 mg/mL (155.71 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.3956 mL	11.9778 mL	23.9555 mL
5 mM	0.4791 mL	2.3956 mL	4.7911 mL
10 mM	0.2396 mL	1.1978 mL	2.3956 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.17 mg/mL (5.20 mM); Clear solution

此方案可获得 ≥ 2.17 mg/mL (5.20 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil

Solubility: ≥ 2.17 mg/mL (5.20 mM); Clear solution

此方案可获得 ≥ 2.17 mg/mL (5.20 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在上海金畔生物科技有限公司网站选购。

参考文献

[1]. Gordon Saxty, et al. Imidazo-condensed bicycles as inhibitors of discoidin domain receptors (ddrs)

Kinase Assay ^[1]	Europium Kinase binding assay is used. Compounds (DDR Inhibitor, etc.) are incubated with 0.5 nM DDR1 or 0.25 nM DDR2 for 1 hour at room temperature in low volume black 384 well assay plates containing 5 nM or 10 nM Kinase Tracer 178 respectively and 2 nM Europium labelled anti-GST antibody in assay buffer (50 mM HEPES pH 7.5, 10 mM MgCl₂, 1 mM EGTA and 0.01 % BRIJ35). The ratio of fluorescence emission 665 nm/615 nm after excitation at 340 nm is obtained. IC₅₀ values are determined from dose-response plots using nonlinear least-squares analysis^[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Gordon Saxty, et al. Imidazo-condensed bicycles as inhibitors of discoidin domain receptors (ddrs)

Kinase Assay
^[1]

Europium Kinase binding assay is used. Compounds (DDR Inhibitor, etc.) are incubated with 0.5 nM DDR1 or 0.25 nM DDR2 for 1 hour at room temperature in low volume black 384 well assay plates containing 5 nM or 10 nM Kinase Tracer 178 respectively and 2 nM Europium labelled anti-GST antibody in assay buffer (50 mM HEPES pH 7.5, 10 mM MgCl₂, 1 mM EGTA and 0.01 % BRIJ35). The ratio of fluorescence emission 665 nm/615 nm after excitation at 340 nm is obtained. IC₅₀ values are determined from dose-response plots using nonlinear least-squares analysis^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Gordon Saxty, et al. Imidazo-condensed bicycles as inhibitors of discoidin domain receptors (ddrs)

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