NSC-70220 is a selective and allosteric SOS1 inhibitor. NSC-70220 inhibits allosteric site activation, and partially inhibited catalytic site activation. NSC-70220 has an anticancer effect^[1].

SOS1^[1].

The growth inhibitory rate (IR)=(average OD value in the control group-average OD value in the treatment group)/average OD value in the control group×100% are compared. Treatments with NSC-70220 at 40 μM and NSC-658497 at 30 μM result in growth inhibitory rate of 29% and 8% respectively. The combination treatment results in growth inhibitory rate of 54%, showing the synergistic effect of NSC-70220 and NSC-658497 on cell growth inhibition. It is showed that the treatment with NSC-658497 at 40 μM for 2 hours alone or in combìnation with the treatment with 40 μM of NSC-70220 overnight resulted in lower expression levels of P-AKT and P-ERK. The treatment wlth 40 μM of NSC-70220 overnight resulted in lower expression levels of P-ERK^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

325.36

C₂₂H₁₅NO₂

4551-00-2

Room temperature in continental US; may vary elsewhere.

Powder	-20°C	3 years
	4°C	2 years

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vitro: 

DMSO : 12.5 mg/mL (38.42 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 1.25 mg/mL (3.84 mM); Clear solution

  此方案可获得 ≥ 1.25 mg/mL (3.84 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 1.25 mg/mL (3.84 mM); Clear solution

  此方案可获得 ≥ 1.25 mg/mL (3.84 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. ARYL SULFONOHYDRAZIDES. WO 2016077793A1.

MIA paca-2 cells (KRAS G12 C) are subjected to a 3-day proliferation assay following treatment with SOS1-IN-1 (NSC-70220: 40 μM), treatment with NSC-658497 (30 μM) and treatment with a combination of SOS1-IN-1 (40 μM) and NSC-658497 (30 μM). DMSO is used as the negative control^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. ARYL SULFONOHYDRAZIDES. WO 2016077793A1.