Bak BH3 | 赛默飞Alfa aesar官网

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Bak BH3

Bak BH3 是一种多肽，源于 Bak 蛋白的 BH3 结构域，在细胞中，能够抑制 Bcl-xL 的活性。

Bak BH3 Chemical Structure

规格	价格	是否有货
1 mg	￥1100	询问价格 & 货期
5 mg	￥3300	询问价格 & 货期
10 mg	￥5500	询问价格 & 货期

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生物活性

Bak BH3 is derived from the BH3 domain of Bak, can antagonize the function of Bcl-xL in cells.

IC₅₀ & Target^[1]

Bcl-xL

体外研究
(In Vitro)

Bak BH3 peptide antagonize the protective effects of microinjected Bcl-xL in α-Fas-treated HeLa cells, whereas a mutant Bak BH3 peptide that no longer binds Bcl-xL is inactive^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1724.90

Formula

C₇₂H₁₂₅N₂₅O₂₄

Sequence

Gly-Gln-Val-Gly-Arg-Gln-Leu-Ala-Ile-Ile-Gly-Asp-Asp-Ile-Asn-Arg

Sequence Shortening

GQVGRQLAIIGDDINR

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献

[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

Cell Assay ^[1]	Cells are plated in complete DMEM in 96-well tissue culture plates at 4×10³/well. After 24 h, cells are washed with PBS and treated with peptides (50 μM) in SF-DMEM. Cell viability is determined by staining unfixed cells with calcein AM/ethidium homodimer, followed by microscopic analysis of cell staining and cellular morphology on a Nikon Diaphot 300 inverted microscope equipped with a fluorescence module. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

Cell Assay
^[1]

Cells are plated in complete DMEM in 96-well tissue culture plates at 4×10³/well. After 24 h, cells are washed with PBS and treated with peptides (50 μM) in SF-DMEM. Cell viability is determined by staining unfixed cells with calcein AM/ethidium homodimer, followed by microscopic analysis of cell staining and cellular morphology on a Nikon Diaphot 300 inverted microscope equipped with a fluorescence module.

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

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