13-Methyltetradecanoic acid (13-MTD), a saturated branched-chain fatty acid with potent anticancer effects. 13-Methyltetradecanoic acid induces apoptosis in many types of human cancer cells[1][2].
体外研究 (In Vitro)
13-Methyltetradecanoic acid (13-MTD; 0-140 μg/mL; 12-24 hours) inhibits cell viability and proliferation in human bladder cancer cells by inducing apoptosis[1]. 13-Methyltetradecanoic acid (13-MTD; 70 μg/mL; 2-48 hours) treatments results in significant accumulation of cells with sub-G1 DNA content in a time-dependent manner, with the proportion of sub-G1 phase DNA content ranging from 9.25% to 85.3% over 2-48 hours[1]. 13-Methyltetradecanoic acid (13-MTD; 70 μg/mL; 2-24 hours) down-regulates Bcl-2 and up-regulates Bax. This promotes mitochondrial dysfunction, leading to the release of cytochrome c from the mitochondria to the cytoplasm, as well as the proteolytic activation of caspases. 13-Methyltetradecanoic acid down-regulates AKT phosphorylation and activates phosphorylation of p38 and c-Jun N-terminal kinase (JNK)[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Inhibition of cell viability in a dose- and time-dependent manner.
Cell Cycle Analysis[1]
Cell Line:
Bladder cancer cell lines T24, 5637, and UM-UC-3
Concentration:
70 μg/mL
Incubation Time:
2 hours, 8 hours, 24 hours, or 48 hours
Result:
Resulted in significant accumulation of cells with sub-G1 DNA content in a time-dependent manner.
Western Blot Analysis[1]
Cell Line:
T24, 5637, and UM-UC-3 cells
Concentration:
70 μg/mL
Incubation Time:
2 hours, 8 hours, 24 hours
Result:
Down-regulated Bcl-2 and up-regulated Bax, and down-regulated AKT phosphorylation and activated phosphorylation of p38 and c-Jun N-terminal kinase (JNK).
体内研究 (In Vivo)
13-Methyltetradecanoic acid (13-MTD; 70 mg/kg/day; oral gavage; daily; for 30 days) significantly suppresses tumor growth in a xenograft model[2].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
BALB/C nude mice injected with Jurkat lymphoma cells[2]
Dosage:
70 mg/kg/day
Administration:
Oral gavage; daily; for 30 days
Result:
Effectively inhibited the growth in vivo in a xenograft model.
分子量
242.40
Formula
C15H30O2
CAS 号
2485-71-4
中文名称
13-甲基十四烷酸
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Tianxin Lin, et al. 13-Methyltetradecanoic acid induces mitochondrial-mediated apoptosis in human bladder cancer cells. Urol Oncol. May-Jun 2012;30(3):339-45.
[2]. Qingqing Cai, et al. 13-methyltetradecanoic acid exhibits anti-tumor activity on T-cell lymphomas in vitro and in vivo by down-regulating p-AKT and activating caspase-3. PLoS One. 2013 Jun 7;8(6):e65308.
