JW67

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

JW67 

JW67 抑制典型 Wnt 信号,其 IC50 值为 1.17 μM。JW67 影响由 β- 连环蛋白/GSK-3β/AXIN/APC/CK1 组成的多蛋白复合物,该复合物可快速降低活性 β- 连环蛋白,随后下调 Wnt 靶基因。JW67 也抑制结直肠癌细胞生长。

JW67

JW67 Chemical Structure

CAS No. : 442644-28-2

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生物活性

JW67 inhibits the canonical Wnt signaling with an IC50 of 1.17μM[1]. JW67 affects the multiprotein complex consisting of β-catenin/GSK-3β/AXIN/APC/CK1 that rapidly reduces active β-catenin with a subsequent downregulation of Wnt target genes. JW67 also inhibits colorectal cancer cell growth[1].

IC50 & Target

IC50: 1.17μM (Wnt signaling)[1]

体外研究
(In Vitro)

JW67 (1 μM; 24 hours) increases concentration of AXIN2 and decreases the active form of β-catenin[1].
JW67 affects the expression of Wnt target genes and reduces cell growth of colon cancer cell lines[1].
JW67 shows a concentrationdependent reduction in proliferation with a GI50 value of 7.8 μM[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: SW480 cells
Concentration: 1 μM
Incubation Time: 24 hours
Result: Increased the AXIN2 expression.

RT-PCR[1]

Cell Line: SW480 cells
Concentration: 10 or 25μM
Incubation Time: 72 hours
Result: Reduced growth of SW480 CRC cells in vitro by inhibiting cell-cycle progression at the G1/S.

分子量

394.38

Formula

C21H18N2O6

CAS 号

442644-28-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Waaler J, et al. Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. Cancer Res. 2011;71(1):197-205.

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