Malantide TFA is a synthetic dodecapeptide derived from the site phosphorylated by cAMP-dependent protein kinase (PKA) on the β-subunit of phosphorylase kinase. Malantide TFA is a highly specific substrate for PKA with a K_m of 15 μM and shows protein inhibitor (PKI) inhibition >90% substrate phosphorylation in various rat tissue extracts^[1]. Malantide TFA is also an efficient substrate for PKC with a K_m of 16 μM^[2].

Km: 15 μM (PKA)^[1], 16 μM (PKC)^[2], 233 μM (PKG)^[1]

The K_m values of Malantide are 15 μM and 223 μM for PKA and PKG, respectively. The V_max values are 23.8 units/mg and 6.6 units/mg for for PKA and PKG, respectively^[1].
A statistically significant effect of isoprenaline on the activity ratio of guinea-pig heart was only: observed with Malantide^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

1747.91

C₇₄H₁₂₅F₃N₂₂O₂₃

Arg-Thr-Lys-Arg-Ser-Gly-Ser-Val-Tyr-Glu-Pro-Leu-Lys-Ile

RTKRSGSVYEPLKI

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. K J Murray, et al. Use of a Synthetic Dodecapeptide (Malantide) to Measure the Cyclic AMP-dependent Protein Kinase Activity Ratio in a Variety of Tissues. Biochem J. 1990 May 1;267(3):703-8.

  [2]. Z H Zhao, et al. Characterization of a New Substrate for Protein Kinase C: Assay by Continuous Fluorometric Monitoring and High Performance Liquid Chromatography. Biochem Biophys Res Commun. 1991 May 15;176(3):1454-61.