Fz7-21 TFA(Synonyms: Ac-LPSDDLEFWCHVMY-NH2 TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Fz7-21 TFA (Synonyms: Ac-LPSDDLEFWCHVMY-NH2 TFA) 纯度: 99.87%

Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA 是 Frizzled 7 (FZD 7) 受体的肽拮抗剂,选择性地与 FZD7 CRD 亚类结合。在人和小鼠 FZD7 CRD 中的 EC50 值分别为 58 和 34 nM。Fz7-21 TFA 对外源性 WNT3A (IC50=100 nM) 刺激和转染 WNT3A 或 WNT1 的 HEK293 细胞 Wnt/β-catenin 信号传导有损伤。Fz7-21 TFA 还阻断了 WNT3A 介导的 β-catenin 在小鼠 L 细胞中的稳定作用 (IC50=50 nM)。

Fz7-21 TFA(Synonyms: Ac-LPSDDLEFWCHVMY-NH2 TFA)

Fz7-21 TFA Chemical Structure

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  • Peptide Library

生物活性

Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA , a peptide antagonist of Frizzled 7 (FZD 7) receptors, selectively binds to FZD7 CRD subclass. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 impairs Wnt/β-catenin signaling in HEK293 cells stimulated with exogenous WNT3A (IC50=100 nM) or transfected with a construct expressing WNT3A or WNT1. Fz7-21 also blocks WNT3A-mediated stabilization of β-catenin in mouse L cells (IC50=50 nM)[1].

IC50 & Target

EC50: 58 nM (human FZD7 CRD), 34 nM (mouse FZD7 CRD)[1]

分子量

1910.07

Formula

C85H115N18F3O25S2

Sequence Shortening

Ac-LPSDDLEFWCHVMY-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 33.33 mg/mL (17.45 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5235 mL 2.6177 mL 5.2354 mL
5 mM 0.1047 mL 0.5235 mL 1.0471 mL
10 mM 0.0524 mL 0.2618 mL 0.5235 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (1.31 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (1.31 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (1.31 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.31 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Nile AH, et al. Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells. Nat Chem Biol. 2018 Sep;14(9):902.

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Endothelin-2 (49-69), human TFA(Synonyms: Endothelin-2 (49-69) (human, canine) TFA; Human endothelin-2 TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Endothelin-2 (49-69), human TFA (Synonyms: Endothelin-2 (49-69) (human, canine) TFA; Human endothelin-2 TFA) 纯度: 99.82%

Endothelin-2 (49-69), human (TFA) (Endothelin-2 (49-69) (human, canine) (TFA)) 是由 21 个氨基酸组成的血管活性肽,可与 G 蛋白连接的跨膜受体,ET-RAET-RB 结合。

Endothelin-2 (49-69), human TFA(Synonyms: Endothelin-2 (49-69) (human, canine) TFA; Human endothelin-2 TFA)

Endothelin-2 (49-69), human TFA Chemical Structure

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500 μg ¥3120 In-stock

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Endothelin-2 (49-69), human TFA 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Endothelin-2 (49-69), human (TFA) (Endothelin-2 (49-69) (human, canine) (TFA)) is a 21-amino acid vasoactive peptide that binds to G-protein-linked transmembrane receptors, ET-RA and ET-RB.

体外研究
(In Vitro)

At nanomolar concentrations, Endothelin-2 exhibits a strong and long-lasting vasoconstrictor activity characteristic of endothelin (EC50=0.52 nM)[1].
Endothelin-2 is a chemoattractant for macrophages and THP-1 monocytic cells, but not for freshly isolated monocytes. The chemotactic response to Endothelin-2 shows a typical bell-shaped response curve. Experiments with endothelin receptor antagonists shows that migration to Endothelin-2 is mediated via the ET-RB receptor. Endothelin-2 expression by tumors may modulate the behavior of macrophages such that activated cells accumulate in areas of hypoxia[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Two distinct phases of pressor effects are typically seen in response to Endothelin-2: the early phase immediately follows the depressor response and dominates during the next 3-10 min, whereas the late pressor effect develops 10-20 min after the injection and lasts >1 hr[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2660.94

Formula

C117H161N26F3O34S4

Sequence

Cys-Ser-Cys-Ser-Ser-Trp-Leu-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp (Disulfide bridge: Cys1-Cys15, Cys3-Cys11)

Sequence Shortening

CSCSSWLDKECVYFCHLDIIW (Disulfide bridge: Cys1-Cys15, Cys3-Cys11)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

H2O : 6.67 mg/mL (2.51 mM; ultrasonic and adjust pH to 3 with HCl)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.3758 mL 1.8790 mL 3.7581 mL
5 mM
10 mM

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Grimshaw MJ, et al. Endothelin-2 is a macrophage chemoattractant: implications for macrophage distribution in tumors. Eur J Immunol. 2002 Sep;32(9):2393-400.

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Cyclic MKEY TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Cyclic MKEY TFA 

Cyclic MKEY TFA 是 CXCL4-CCL5 异二聚体形成的合成环肽抑制剂,其通过介导炎症来防止动脉粥样硬化和主动脉瘤形成。Cyclic MKEY TFA 还可以预防小鼠中风引起的脑损伤。

Cyclic MKEY TFA

Cyclic MKEY TFA Chemical Structure

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生物活性

Cyclic MKEY TFA is a synthetic cyclic peptide inhibitor of CXCL4-CCL5 heterodimer formation, which protects against atherosclerosis and aortic aneurysm formation by mediating inflammation. Cyclic MKEY TFA also protects against stroke-induced brain injury in mice[1][2].

IC50 & Target

CXCL4-CCL5[1]

分子量

2646.91

Formula

C115H175N28F3O36S2

Sequence Shortening

CKEYFYTSSKSSNLAVVFVTRC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Fan Y,et al. MKEY, a Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, Protects Against Stroke in Mice. J Am Heart Assoc. 2016 Sep 15;5(9). pii: e003615.

    [2]. Iida Y, et al. Peptide inhibitor of CXCL4-CCL5 heterodimer formation, MKEY, inhibits experimental aortic aneurysm initiation and progression. Arterioscler Thromb Vasc Biol. 2013 Apr;33(4):718-26.

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Ac-IEVDIDVEH TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Ac-IEVDIDVEH TFA 

Ac-IEVDIDVEH TFA 是一个短肽序列,末端是 Ac。

Ac-IEVDIDVEH TFA

Ac-IEVDIDVEH TFA Chemical Structure

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生物活性

Ac-IEVDIDVEH TFA is a short peptide sequence with Ac at the end.

分子量

1224.19

Formula

C50H76F3N11O21

Sequence

Ac-Ile-Glu-Val-Asp-Ile-Asp-Val-Glu-His

Sequence Shortening

Ac-IEVDIDVEH

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

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Ac-IEVDIDVE TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Ac-IEVDIDVE TFA 

Ac-IEVDIDVE TFA 是一个短肽序列,末端是 Ac。

Ac-IEVDIDVE TFA

Ac-IEVDIDVE TFA Chemical Structure

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生物活性

Ac-IEVDIDVE TFA is a short peptide sequence with Ac at the end.

分子量

1087.06

Formula

C44H69F3N8O20

Sequence

Ac-Ile-Glu-Val-Asp-Ile-Asp-Val-Glu

Sequence Shortening

Ac-IEVDIDVE

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

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Ac-IEVDIDV TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Ac-IEVDIDV TFA 

Ac-IEVDIDV TFA 是一个短肽序列,末端是 Ac。

Ac-IEVDIDV TFA

Ac-IEVDIDV TFA Chemical Structure

规格 是否有货
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生物活性

Ac-IEVDIDV TFA is a short peptide sequence with Ac at the end.

分子量

957.94

Formula

C39H62F3N7O17

Sequence

Ac-Ile-Glu-Val-Asp-Ile-Asp-Val

Sequence Shortening

Ac-IEVDIDV

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

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Ac-IEVDID TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Ac-IEVDID TFA 

Ac-IEVDID TFA 是一个短肽序列,末端是 Ac。

Ac-IEVDID TFA

Ac-IEVDID TFA Chemical Structure

规格 是否有货
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250 mg   询价  
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生物活性

Ac-IEVDID TFA is a short peptide sequence with Ac at the end.

分子量

858.81

Formula

C34H53F3N6O16

Sequence

Ac-Ile-Glu-Val-Asp-Ile-Asp

Sequence Shortening

Ac-IEVDID

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

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Cilengitide TFA(Synonyms: EMD 121974 TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Cilengitide TFA (Synonyms: EMD 121974 TFA) 纯度: 98.85%

Cilengitide 是有效的,选择性的 αvβ3 和 αvβ5 受体整合素抑制剂,IC50 分别为 4 nM 和 79 nM。

Cilengitide TFA(Synonyms: EMD 121974 TFA)

Cilengitide TFA Chemical Structure

CAS No. : 199807-35-7

规格 价格 是否有货 数量
10 mM * 1 mL in Water ¥2320 In-stock
5 mg ¥1500 In-stock
10 mg ¥2500 In-stock
25 mg ¥4500 In-stock
50 mg   询价  
100 mg   询价  

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生物活性

Cilengitide is a potent and selective integrin inhibitor for αvβ3 and αvβ5 receptor, with IC50 values of 4 nM and 79 nM, respectively.

IC50 & Target

IC50: 4/79 nM (αvβ3vβ5)[1].

体外研究
(In Vitro)

Cilengitide (EMD 121974) is the αvβ3 and αvβ5 integrin receptor antagonist. In cell adhesion studies assessing the human melanoma M21 or UCLA-P3 human lung carcinoma cell lines, Cilengitide inhibits integrin-mediated binding to vitronectin with IC50s of 0.4 and 0.4 μM[1]. In vitro treatment of Cilengitide, at a concentration greater than 1 µM, shows concentration- and time-dependent cytotoxic effects [2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

In nude mice bearing M21-L melanoma tumors, Cilengitide dose i.p. at 10, 50, and 250 μg three times per week demonstrate inhibition of tumor growth with a reduction in both tumor volume (55%, 75%, and 89%, respectively) and tumor weight (23%, 38%, and 61%, respectively), when compared to controls[2]. In the rat model studied, the systemic pharmacokinetics of i.p. Cilengitide are not affected by ILP with Cilengitide alone or ILP with Cilengitide plus Melphalan, TNF or both. Systemic Cilengitide levels reach around 20 µg/mL (approximately 35 µM) within 10 min of i.p. administration and continued to rise to approximately 40 µg/mL (approximately 70 µM) in the first hour. Thereafter Cilengitide levels in serum drop with an elimination half-life of 2.1 hr[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

702.68

Formula

C29H41F3N8O9

CAS 号

199807-35-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 25 mg/mL (35.58 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4231 mL 7.1156 mL 14.2312 mL
5 mM 0.2846 mL 1.4231 mL 2.8462 mL
10 mM 0.1423 mL 0.7116 mL 1.4231 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Hariharan S, et al. Assessment of the biological and pharmacological effects of the alpha nu beta3 and alpha nu beta5 integrinreceptor antagonist, Cilengitide (EMD 121974), in patients with advanced solid tumors. Ann Oncol. 2007 Aug;18(8):1400-7.

    [2]. Kim YH, et al. Combination therapy of cilengitide with belotecan against experimental glioblastoma. Int J Cancer. 2013 Aug 1;133(3):749-56.

    [3]. Ten Hagen TL, et al. The αVβ3/αVβ5 integrin inhibitor cilengitide augments tumor response to melphalan isolated limb perfusion in a sarcoma model. Int J Cancer. 2012 Nov 13.

Cell Assay
[2]

The cytotoxicity of the two drugs, Belotecan and Cilengitide, is measured by the Cell Counting Kit-8 (CCK-8). U87MG and U251MG cells are seeded in 96 well plates at a density of 4×103 cells per well to allow for adhesion overnight. After this, the cells are treated with Cilengitide at a concentration of 0, 0.1, 0.5, 1, 5 and 25 µM and Belotecan at a concentration of 0, 6.25, 12.5, 25, 50 and 100 nM. All possible combinations of concentrations are used to assess the combined therapeutic effect of Cilengitide and Belotecan. After 3 days, 10 µL of the CCK-8 solution is added to each well of the plate, and the plate is incubated for 3 hr in the incubator (37°C; 5% CO2)[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2][3]

Mice[2]
Male Balb/c-nu mice, at 8 weeks of age, are randomly assigned to four groups: control (n=10), Cilengitide (n=10), Belotecan (n=10) and combination (n=10). Cilengitide is administered intraperitoneally at a dose of 20 mg/kg daily and the Belotecan at a dose of 10 mg/kg every 4 days. The drug treatments began 7 days after the implantation of tumor cells for 16 days. Half of the animals are sacrificed 1 month after the implantation of the tumor cells for tumor volume analysis and the rest of the animals are observed for another 2 months to analyze survival. The death of the animals is defined as a weight reduction of over 25% of the initial weight or an unexpected sudden death beforehand[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Hariharan S, et al. Assessment of the biological and pharmacological effects of the alpha nu beta3 and alpha nu beta5 integrinreceptor antagonist, Cilengitide (EMD 121974), in patients with advanced solid tumors. Ann Oncol. 2007 Aug;18(8):1400-7.

    [2]. Kim YH, et al. Combination therapy of cilengitide with belotecan against experimental glioblastoma. Int J Cancer. 2013 Aug 1;133(3):749-56.

    [3]. Ten Hagen TL, et al. The αVβ3/αVβ5 integrin inhibitor cilengitide augments tumor response to melphalan isolated limb perfusion in a sarcoma model. Int J Cancer. 2012 Nov 13.

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Ac-VDID TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Ac-VDID TFA 

Ac-VDID TFA 是一个短肽序列,末端是 Ac。

Ac-VDID TFA

Ac-VDID TFA Chemical Structure

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生物活性

Ac-VDID TFA is a short peptide sequence with Ac at the end.

分子量

616.54

Formula

C23H35F3N4O12

Sequence

Ac-Val-Asp-Ile-Asp

Sequence Shortening

Ac-VDID

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

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Thalidomide-O-amido-PEG3-C2-NH2 TFA(Synonyms: Cereblon Ligand-Linker Conjugates 3 TFA; E3 ligase Ligand-Linker Conjugates 14 TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Thalidomide-O-amido-PEG3-C2-NH2 TFA (Synonyms: Cereblon Ligand-Linker Conjugates 3 TFA; E3 ligase Ligand-Linker Conjugates 14 TFA) 纯度: 99.55%

Thalidomide-O-amido-PEG3-C2-NH2 TFA 是一种合成的 E3 连接酶配体-linker 偶联物,包含基于 Thalidomide 的 cereblon 配体和 3 个单元的 PEG linker。

Thalidomide-O-amido-PEG3-C2-NH2 TFA(Synonyms: Cereblon Ligand-Linker Conjugates 3 TFA; E3 ligase Ligand-Linker Conjugates 14 TFA)

Thalidomide-O-amido-PEG3-C2-NH2 TFA Chemical Structure

CAS No. : 1957236-21-3

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Free Sample (0.1-0.5 mg)   Apply now  
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500 mg ¥13500 In-stock
1 g ¥25000 In-stock
2 g ¥35000 In-stock
5 g   询价  
10 g   询价  

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生物活性

Thalidomide-O-amido-PEG3-C2-NH2 TFA is a synthesized E3 ligase ligand-linker conjugate that incorporates the Thalidomide based cereblon ligand and 3-unit PEG linker used in PROTAC technology.

IC50 & Target

Cereblon

 

分子量

620.53

Formula

C25H31F3N4O11

CAS 号

1957236-21-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 50 mg/mL (80.58 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6115 mL 8.0576 mL 16.1153 mL
5 mM 0.3223 mL 1.6115 mL 3.2231 mL
10 mM 0.1612 mL 0.8058 mL 1.6115 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. BRADNER, James, et al. METHODS TO INDUCE TARGETED PROTEIN DEGRADATION THROUGH BIFUNCTIONAL MOLECULES. WO2017024317A2.

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Gp100 (25-33), human TFA(Synonyms: Hgp100 (25-33) (TFA))

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Gp100 (25-33), human TFA (Synonyms: Hgp100 (25-33) (TFA)) 纯度: 99.60%

Gp100 (25-33), human TFA (Hgp100 (25-33) TFA) 是人类黑色素瘤抗原的 25-33 个氨基酸片段。Gp100 (25-33), human TFA 是一种受 H-2Db 限制并被 T 细胞识别的 9 个氨基酸 (AA) 表位。

Gp100 (25-33), human TFA(Synonyms: Hgp100 (25-33) (TFA))

Gp100 (25-33), human TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥900 In-stock
5 mg ¥2700 In-stock
10 mg ¥4200 In-stock
50 mg   询价  
100 mg   询价  

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Gp100 (25-33), human TFA 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Gp100 (25-33), human TFA (Hgp100 (25-33) TFA) is the amino acids 25-33 fragment of the human melanoma antigen. It is a 9-amino acid (AA) epitope restricted by H-2Db and recognized by the T cells[1].

分子量

1269.33

Formula

C54H83F3N16O16

Sequence

Lys-Val-Pro-Arg-Asn-Gln-Asp-Trp-Leu

Sequence Shortening

KVPRNQDWL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

H2O : 100 mg/mL (78.78 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7878 mL 3.9391 mL 7.8782 mL
5 mM 0.1576 mL 0.7878 mL 1.5756 mL
10 mM 0.0788 mL 0.3939 mL 0.7878 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Overwijk WW, et al. gp100/pmel 17 is a murine tumor rejection antigen: induction of “self”-reactive, tumoricidal T cellsusing high-affinity, altered peptide ligand. J Exp Med. 1998 Jul 20;188(2):277-86.

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R18 TFA(Synonyms: PHCVPRDLSWLDLEANMCLP TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

R18 TFA (Synonyms: PHCVPRDLSWLDLEANMCLP TFA) 纯度: 98.35%

R18 TFA 是 14-3-3 的肽拮抗剂,其KD 值为 70-90 nM。R18 可有效阻断14-3-3 与其配体Raf-1激酶的结合,可有效地消除14-3-3对磷酸酶诱导的Raf-1失活的保护作用。

R18 TFA(Synonyms: PHCVPRDLSWLDLEANMCLP TFA)

R18 TFA Chemical Structure

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10 mg ¥6000 In-stock
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100 mg   询价  

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R18 TFA 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

R18 TFA is a peptide antagonists of 14-3-3, with a KD of 70-90 nM. R18 efficiently blocks the binding of 14-3-3 to the kinase Raf-1, a physiological ligand of 14-3-3, and effectively abolished the protective role of 14-3-3 against phosphatase-induced inactivation of Raf-1[1].

分子量

2423.73

Formula

C103H158F3N27O31S3

Sequence

Pro-His-Cys-Val-Pro-Arg-Asp-Leu-Ser-Trp-Leu-Asp-Leu-Glu-Ala-Asn-Met-Cys-Leu-Pro

Sequence Shortening

PHCVPRDLSWLDLEANMCLP

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : < 0.1 mg/mL (ultrasonic) (insoluble)

参考文献
  • [1]. B Wang, et al. Isolation of high-affinity peptide antagonists of 14-3-3 proteins by phage display. Biochemistry. 1999 Sep 21;38(38):12499-504.

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c-Myc Peptide TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

c-Myc Peptide TFA  纯度: 95.63%

c-Myc Peptide (TFA) 是一种合成肽,与人 c-myc 蛋白的 C-末端氨基酸 (410-419) 相对应,参与调控与生长有关的基因的转录过程。

c-Myc Peptide TFA

c-Myc Peptide TFA Chemical Structure

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1 mg ¥600 In-stock
5 mg ¥1800 In-stock
10 mg ¥2800 In-stock
50 mg   询价  
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c-Myc Peptide TFA 相关产品

相关化合物库:

  • Bioactive Compound Library Plus

生物活性

c-Myc Peptide (TFA) is a synthetic peptide corresponding to the C-terminal amino acids (410-419) of human c-myc protein, and participates in regulation of growth-related gene transcription.

体外研究
(In Vitro)

C-myc plays a critical role in the growth of breast cancer cells[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1317.32

Formula

C2HF3O2

Sequence

Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu

Sequence Shortening

EQKLISEEDL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 50 mg/mL (37.96 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7591 mL 3.7956 mL 7.5912 mL
5 mM 0.1518 mL 0.7591 mL 1.5182 mL
10 mM 0.0759 mL 0.3796 mL 0.7591 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Watson PH, et al. Inhibition of c-myc expression by phosphorothioate antisense oligonucleotide identifies a critical role for c-myc in the growth of human breast cancer. Cancer Res. 1991 Aug 1;51(15):3996-4000.

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FTI 276 TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

FTI 276 TFA  纯度: 98.06%

FTI-276 是一种有效的 protein farnesyl transferase (PFT) 抑制剂, 作用于恶性疟原虫和人类的 IC50 值分别为 0.9 nM 和 0.5 nM。

FTI 276 TFA

FTI 276 TFA Chemical Structure

CAS No. : 1217471-51-6

规格 价格 是否有货
10 mM * 1 mL in DMSO ¥4820 询问价格 & 货期
5 mg ¥4000 询问价格 & 货期
10 mg ¥6800 询问价格 & 货期
25 mg ¥13500 询问价格 & 货期
50 mg ¥22000 询问价格 & 货期

* Please select Quantity before adding items.

FTI 276 TFA 相关产品

相关化合物库:

  • Covalent Screening Library Plus
  • Bioactive Compound Library Plus
  • Metabolism/Protease Compound Library
  • Anti-Cancer Compound Library
  • Covalent Screening Library
  • Anti-Pancreatic Cancer Compound Library

生物活性

FTI-276 is a protein farnesyl transferase (PFT) inhibitor with IC50s of 0.9 nM and 0.5 nM for Plasmodium falciparum and human, respectively[1].

IC50 & Target

IC50: 0.9 nM (PFT, Plasmodium falciparum), 0.5 nM (PFT, human)[1].

分子量

547.61

Formula

C23H28F3N3O5S2

CAS 号

1217471-51-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL (182.61 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8261 mL 9.1306 mL 18.2612 mL
5 mM 0.3652 mL 1.8261 mL 3.6522 mL
10 mM 0.1826 mL 0.9131 mL 1.8261 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.57 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.57 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.57 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.57 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.57 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.57 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Chakrabarti D, et al. Protein farnesyltransferase and protein prenylation in Plasmodium falciparum.J Biol Chem. 2002 Nov 1; 277(44):42066-73. Epub 2002 Aug 22.

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CGGRGD TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

CGGRGD TFA 

CGGRGD TFA,一种以半胱氨酸为 N 端的 RGD 衍生物。CGGRGD TFA 通过固相肽合成技术合成的,PCL 纤维的表面被氨基2-氰基苯并噻唑氨解,然后加入 2-氰基苯并噻唑 (CBT)。

CGGRGD TFA

CGGRGD TFA Chemical Structure

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250 mg   询价  
500 mg   询价  

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生物活性

CGGRGD TFA, a RGD derivative with cysteine as its N-terminal, CGGRGD TFA is synthesized via solid-phase peptide synthesis technique and the surface of PCL fibers is aminolysised by amino 2-cyanobenzothiazole followed by the addition of 2-cyanobenzothiazole (CBT)[1].

体外研究
(In Vitro)

CGGRGD TFA is a characteristic sequence of fifibronectin that binds to the receptors on cell surface. (PCL) is a popular materials for tissue engineering due to its optimal biocompatibility, biodegradability, and thermoplastic characteristics[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

677.61

Formula

C21H34F3N9O11S

Sequence

Cys-Gly-Gly-Arg-Gly-Asp

Sequence Shortening

CGGRGD

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Wenting Zhen, et al.Functionalization of PCL fibrous membrane with RGD peptide

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ACTH (1-17) (TFA)(Synonyms: α1-17-ACTH TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

ACTH (1-17) (TFA) (Synonyms: α1-17-ACTH TFA)

ACTH (1-17) TFA 是促肾上腺皮质激素类似物,是一种有效的人黑皮素受体 (human melanocortin 1 (MC1) receptor) 激动剂,Ki 值为 0.21 nM。

ACTH (1-17) (TFA)(Synonyms: α1-17-ACTH TFA)

ACTH (1-17) (TFA) Chemical Structure

规格 价格 是否有货
1 mg ¥1300 询问价格 & 货期
5 mg ¥4500 询问价格 & 货期
10 mg ¥6500 询问价格 & 货期

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ACTH (1-17) (TFA) 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Neuronal Signaling Compound Library
  • Anti-Cancer Compound Library
  • Endocrinology Compound Library
  • Anti-Obesity Compound Library
  • Targeted Diversity Library

生物活性

ACTH (1-17) TFA, an adrenocorticotropin analogue, is a potent human melanocortin 1 (MC1) receptor agonist with a Ki of 0.21 nM.

IC50 & Target

Ki: 0.21 nM (human MC1 receptor)[1]

体外研究
(In Vitro)

ACTH (1-17) TFA is a potent agonist at the hMC1R. ACTH (1-17) shows high affinity for the hMC1R with a Ki value of 0.21±0.03 nM which is slightly higher than that of 0.13±0.005 nM for alpha-MSH[1]. ACTH (1-17) induces a slight and not significant increase in growth hormone secretion even when micromolar concentrations of the peptide are employed in rat pituitary cultures[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Inhibition of DNA labeling is noted when the ACTH (1-17) is administered at 2 hr after the beginning of the daily dark span when nocturnal animals become active. When administered at this circadian stage, the larger dose in particular is associated with an inhibition of DNA labeling lasting for 24 hr. The inhibitory effect is much shorter when the same dose is injected 4 hr earlier[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2207.43

Formula

C95H145N29O23S.C2HF3O2

Sequence

Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg

Sequence Shortening

SYSMEHFRWGKPVGKKR

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Tsatmali M et al. ACTH1-17 is a more potent agonist at the human MC1 receptor than alpha-MSH. Cell Mol Biol (Noisy-le-grand). 1999 Nov;45(7):1029-34.

    [2]. Ceda GP, et al. The effects of ACTH (1-17) on GH secretion in vitro. Horm Metab Res. 1987 Aug;19(8):361-3.

    [3]. Walker WV, et al. Effect of an adrenocorticotropin analogue, ACTH (1-17), on DNA synthesis in murine metaphyseal bone. Biochem Pharmacol. 1985 Apr 15;34(8):1191-6.

Cell Assay
[1]

Rat pituitary cells are incubated in the presence of varying concentrations of ACTH (1-17) (0.1 nM-1 μM). A significant increase of growth hormone secretion is documented with each concentration[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice[3]

The effects of ACTH (1-17) on the rate of DNA labeling in the metaphyseal bone of CD2F1 mice are tested on a chronopharmacological dosing schedule. Groups of mice that has been conditioned to a 12-hr light/12-hr dark schedule are injected at one of six different timepoints, 4 hr apart during ,a single 24-hr span with either a low (0.021 I.U/kg) or a high (20 I.U./kg) dose of ACTH (1-17). Control groups receive injections of a placebo at corresponding timepoints. Subgroups of mice are injected with [3H]thymidine ([3H]Tdr) to follow the changes in DNA labeling in the proximal tibial metaphysis at 15 min and 2, 4, 8, 12 and 24 hr after ACTH (1-17) or placebo treatment[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Tsatmali M et al. ACTH1-17 is a more potent agonist at the human MC1 receptor than alpha-MSH. Cell Mol Biol (Noisy-le-grand). 1999 Nov;45(7):1029-34.

    [2]. Ceda GP, et al. The effects of ACTH (1-17) on GH secretion in vitro. Horm Metab Res. 1987 Aug;19(8):361-3.

    [3]. Walker WV, et al. Effect of an adrenocorticotropin analogue, ACTH (1-17), on DNA synthesis in murine metaphyseal bone. Biochem Pharmacol. 1985 Apr 15;34(8):1191-6.

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SAH-SOS1A TFA

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

SAH-SOS1A TFA  纯度: 99.37%

SAH-SOS1A TFA 是一种基于肽的 SOS1/KRAS 蛋白相互作用抑制剂。SAH-SOS1A TFA 以纳摩尔亲和力 (EC50=106-175 nM) 与野生型和突变型 KRAS (G12D, G12V, G12C, G12S, Q61H) 结合,直接和独立地阻断核苷酸结合。SAH-SOS1A TFA 损害 KRAS 驱动的癌细胞活力,并通过阻断 KRAS 下游 ERK-MAPK 磷酸化信号级联的机制发挥作用。

SAH-SOS1A TFA

SAH-SOS1A TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ¥4200 In-stock
10 mg ¥7000 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

SAH-SOS1A TFA 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

SAH-SOS1A TFA is a peptide-based SOS1/KRAS protein interaction inhibitor. SAH-SOS1A TFA binds to wild-type and mutant KRAS (G12D, G12V, G12C, G12S, and Q61H) with nanomolar affinity (EC50=106-175 nM). SAH-SOS1A TFA directly and independently blocks nucleotide association. SAH-SOS1A TFA impairs KRAS-driven cancer cell viability and exerts its effects by on-mechanism blockade of the ERK-MAPK phosphosignaling cascade downstream of KRAS[1].

IC50 & Target

KRAS-SOS1

 

KRas G12C

140 nM (EC50)

KRas G12D

109 nM (EC50)

KRas G12V

154 nM (EC50)

KRas G12S

155 nM (EC50)

KRas Q61H

175 nM (EC50)

K-Ras WT

106 nM (EC50)

体外研究
(In Vitro)

SAH-SOS1A TFA (0.625-40 μM; 24 hours) dose-responsively impairs the viability of cancer cells bearing G12D, G12C, G12V, G12S, G13D, and Q61H mutations with IC50 values in the 5- to 15-μM range. Cancer cells expressing wild-type KRAS, such as HeLa and Colo320-HSR cells, are similarly affected[1].
SAH-SOS1A TFA (5-40 μM; 4 hours) dose-responsively inhibits MEK1/2, ERK1/2, and AKT phosphorylation[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Panc 10.05 cells bearing the KRAS G12D mutation
Concentration: 0.625-40 μM
Incubation Time: 24 hours
Result: Dose-responsively impaired the viability of cancer cells bearing KRAS G12D.

Western Blot Analysis [1]

Cell Line: Panc 10.05 cells
Concentration: 5-40 μM
Incubation Time: Indicated doses for 4 h, followed by 15-min stimulation with EGF
Result: Dose-responsively inhibited MEK1/2, ERK1/2, and AKT phosphorylation.

体内研究
(In Vivo)

SAH-SOS1A (0.2 μL of 10 mM solution; injection; 48 hours; abdomens of D. melanogaster Ras85DV12/ActinGS) treatment notably decreases the phosphorylation state of ERK1/2[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2301.55

Formula

C102H160N27F3O30

Sequence

Arg-Arg-Phe-Phe-Gly-Ile-Aaa-Leu-Thr-Asn-Aaa-Leu-Lys-Thr-Glu-Glu-Gly-Asn (Covalent bridge:Aaa7-Aaa11)

Sequence Shortening

RRFFGI{Aaa}LTN{Aaa}LKTEEGN (Covalent bridge:Aaa7-Aaa11)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light, stored under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro: 

H2O : 33.33 mg/mL (14.48 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.4345 mL 2.1724 mL 4.3449 mL
5 mM 0.0869 mL 0.4345 mL 0.8690 mL
10 mM 0.0434 mL 0.2172 mL 0.4345 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Leshchiner ES, et al. Direct inhibition of oncogenic KRAS by hydrocarbon-stapled SOS1 helices. Proc Natl Acad Sci U S A. 2015;112(6):1761-1766.

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KKI-5 (TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

KKI-5 (TFA)  纯度: 99.93%

KKI-5 (TFA) 是一种特异性的组织激肽释放酶 (kallikrein) 抑制剂。KKI-5 (TFA) 可降低乳腺癌细胞浸润。

KKI-5 (TFA)

KKI-5 (TFA) Chemical Structure

规格 价格 是否有货 数量
1 mg ¥750 In-stock
5 mg ¥2250 In-stock
10 mg ¥3950 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

KKI-5 (TFA) 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

KKI-5 (TFA) is a specific inhibitor of tissue kallikrein. KKI-5 (TFA) can attenuate breast cancer cell invasion[1].

IC50 & Target

Kallikrein[1]

分子量

887.90

Formula

C37H56F3N11O11

Sequence

Ac-Pro-Phe-Arg-Ser-Val-Gln-NH2

Sequence Shortening

Ac-PFRSVQ-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 9.09 mg/mL (10.24 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1263 mL 5.6313 mL 11.2625 mL
5 mM 0.2253 mL 1.1263 mL 2.2525 mL
10 mM 0.1126 mL 0.5631 mL 1.1263 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Deshpande MS, et al. Mapping the binding site of tissue kallikrein: preparation and testing of all possible substrate analog inhibitors homologous with the sequence of kininogen between Ser386 and Gln392. J Med Chem. 1992 Aug 21;35(17):3094-102.

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α-Factor Mating Pheromone, yeast TFA(Synonyms: Mating Factor α TFA)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

α-Factor Mating Pheromone, yeast TFA (Synonyms: Mating Factor α TFA)

α-Factor Mating Pheromone, yeast (TFA) 是一种十三肽,由 S. cerevisiae 的 α 细胞分泌产生,通过 Ste2p 受体起作用。

α-Factor Mating Pheromone, yeast TFA(Synonyms: Mating Factor α TFA)

α-Factor Mating Pheromone, yeast TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥800 In-stock
5 mg ¥2800 In-stock
10 mg ¥4800 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

生物活性

α-Factor Mating Pheromone, yeast (TFA) is a tridecapeptide secreted by S. cerevisiae α cells via Ste2p receptor[1].

分子量

1798.02

Formula

C84H115N20F3O19S

Sequence

Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr

Sequence Shortening

WHWLQLKPGQPMY

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. [1]. Naider F, et al. The alpha-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep;25(9):1441-63.

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Gp100 (25-33), mouse TFA(Synonyms: Mgp100 (25-33) (TFA))

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Gp100 (25-33), mouse TFA (Synonyms: Mgp100 (25-33) (TFA)) 纯度: 99.25%

Gp100 (25-33), mouse TFA 序列位于小鼠自身/肿瘤抗原糖蛋白 (Mgp100) 的第25-33个残基。Mgp100 (25-33), mouse是一种参与色素合成的酶,其表位片段在正常黑色素细胞和黑色素瘤细胞中均有表达。

Gp100 (25-33), mouse TFA(Synonyms: Mgp100 (25-33) (TFA))

Gp100 (25-33), mouse TFA Chemical Structure

规格 价格 是否有货 数量
10 mg ¥2850 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Gp100 (25-33), mouse TFA 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Gp100 (25-33), mouse TFA sequence is found in residues 25 to 33 of the mouse self/tumor antigen glycoprotein (mgp100). Mgp100 is an enzyme involved in pigment synthesis, and the epitope fragment is expressed in both normal melanocytes and melanoma cells[1].

分子量

1218.15

Formula

C48H70F3N15O19

Sequence Shortening

EGSRNQDWL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

参考文献
  • [1]. Gal Cafri, et al. Production of LacZ inducible T cell hybridoma specific for human and mouse gp100 25-33 peptides. PLoS One. 2013;8(2):e55583.

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