5′-Fluoroindirubinoxime(Synonyms: 5′-FIO)

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5′-Fluoroindirubinoxime (Synonyms: 5′-FIO) 纯度: ≥98.0%

5′-Fluoroindirubinoxime (compound 13) 是Indirubin (HY-N0117) 的衍生物,是FLT3 的抑制剂,其IC50 值为15 nM。

5

5′-Fluoroindirubinoxime Chemical Structure

CAS No. : 861214-33-7

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥3850 In-stock
10 mg ¥3500 In-stock
50 mg ¥5500 In-stock
100 mg   询价  
200 mg   询价  

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5′-Fluoroindirubinoxime 相关产品

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生物活性

5′-Fluoroindirubinoxime (5’-FIO, compound 13), an Indirubin (HY-N0117) derivative, is a potent FLT3 inhibitor, with an IC50 of 15 nM[1].

IC50 & Target

15 nM (FLT3)[1].

体外研究
(In Vitro)

5′-Fluoroindirubinoxime (5’-FIO, compound 13) exhibits IC50 values of 1.53 μM and 1.27 μM for VEGFR2 and Aurora A, respectively[1].
5′-Fluoroindirubinoxime (5’-FIO) exhibits IC50 values of 12.2 μM, 2.1 μM, 3.4 μM and 5.1 μM in A549, SNU-638, HT-1080 and RK3E-ras cancer cells, respectively. 5′-Fluoroindirubinoxime (5’-FIO) induces the apoptosis in RK3E-ras cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

5′-Fluoroindirubinoxime (5’-FIO, 10 μmol/L/100 μL (~2.95 mg/mL) every other day beginning on day 6, S.C..) exhibits significant anti-tumor activity in rats[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rat tumor model based RK3E-ras cells[2].
Dosage: S.C..
Administration: 10 μmol/L/100 μL (~2.95 mg/mL) every other day beginning on day 6.
Result: Effectively inhibited tumor growth.

分子量

295.27

Formula

C16H10FN3O2

CAS 号

861214-33-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 83.33 mg/mL (282.22 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3867 mL 16.9337 mL 33.8673 mL
5 mM 0.6773 mL 3.3867 mL 6.7735 mL
10 mM 0.3387 mL 1.6934 mL 3.3867 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (7.04 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (7.04 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Choi SJ, et al. Indirubin derivatives as potent FLT3 inhibitors with anti-proliferative activity of acute myeloid leukemic cells. Bioorg Med Chem Lett. 2010 Mar 15;20(6):2033-7.

    [2]. Kim SA, et al. Antitumor activity of novel indirubin derivatives in rat tumor model. Clin Cancer Res. 2007 Jan 1;13(1):253-9.

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