740 Y-P TFA(Synonyms: 740YPDGFR TFA; PDGFR 740Y-P TFA)

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740 Y-P TFA (Synonyms: 740YPDGFR TFA; PDGFR 740Y-P TFA)

740 Y-P TFA (740YPDGFR; PDGFR 740Y-P) 是一个有效的,具有细胞渗透性的 PI3K 激活剂。740 Y-P TFA 很容易结合含有 p85 的 N- 和 C- 末端 SH2 结构域的 GST 融合蛋白,但不能单独结合 GST。

740 Y-P TFA(Synonyms: 740YPDGFR TFA; PDGFR 740Y-P TFA)

740 Y-P TFA Chemical Structure

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740 Y-P TFA 的其他形式现货产品:

740 Y-P

生物活性

740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone[1].

体外研究
(In Vitro)

740 Y-P TFA (50 μg/ml; 48 hours) specificly stimulates mitogenesis in medium is better than EGF or FGF at stimulating entry into S-phase, it shows the percentage of cells in S-phase for 48.3% in C2 cells. Additionally, LY294002 or wortmannin potently inhibits the mitogenic response stimulated by the 740 Y-P TFA peptide[1].
740 Y-P TFA (1 μg/mL) stimulates mitogenesis at the lowest concentration tested. The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 μg/mL. 740Y-P to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[1].
740 Y-P TFA (30 μM; 24 hours) remarkably inhibits the level of LC3-II/LC3-I in GO-induced PC12 cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: PC12 cells
Concentration: 30 μM
Incubation Time: 24 hours
Result: Inhibited the protein expression of LC3-II.

分子量

3384.72

Formula

C143H223N43F3O41PS3

Sequence Shortening

RQIKIWFQNRRMKWKKSDGG-{PO2Y}-MDMS

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Derossi D, et al. Stimulation of mitogenesis by a cell-permeable PI 3-kinase binding peptide.

    [2]. Xiaoli Feng, et al. Graphene Oxide Induces p62/SQSTM-dependent Apoptosis Through the Impairment of Autophagic Flux and Lysosomal Dysfunction in PC12 Cells. Acta Biomater. 2018 Nov;81:278-292.

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