C8-Ceramide(Synonyms: N-辛酰基-D-神经鞘氨醇; N-Octanoyl-D-erythro-sphingosine)

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C8-Ceramide (Synonyms: N-辛酰基-D-神经鞘氨醇; N-Octanoyl-D-erythro-sphingosine) 纯度: ≥98.0%

C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) 是一种细胞渗透性的内源性神经酰胺类似物。C8-Ceramide 具有具有抗增殖特性，能作为化疗试剂。C8-Ceramide 能刺激树突状细胞促进 T 细胞对病毒感染的反应。C8-Ceramide 在体外能轻微地诱导 PKC 活化。

C8-Ceramide(Synonyms: N-辛酰基-D-神经鞘氨醇; N-Octanoyl-D-erythro-sphingosine)

C8-Ceramide Chemical Structure

CAS No. : 74713-59-0

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生物活性

C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable analog of naturally occurring ceramides. C8-Ceramide has anti-proliferation properties and acts as a potent chemotherapeutic agent. C8-Ceramide stimulates dendritic cells to promote T cell responses upon virus infections. C8-Ceramide induces slight activation of protein kinase (PKC) in vitro[1][2][3][4].

IC50 & Target[2][4][5]

PKC

 

apoptosis

 

autophagy

 

体外研究
(In Vitro)

C8-ceramide (3 μM; 48 hours) irreversibly reduces tumor-cell proliferation and induces morphological changes[1].
C8-ceramide can induce necrosis-like cell death, but does not induce caspase-dependent cleavage of PARP (biochemical marker of apoptosis) in human cervical tumor cells[1].
C8-ceramide may increase the endogenous ROS level (10-30 µM; 24 hours) by regulating the switch of SOD1 and SOD2, causing the anti-proliferation (10-50 µM; 24 hours), and consequently triggering the apoptosis (10-50 µM; 48 hours) of NSCLC H1299 cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: CALO cells, INBL cells, HeLa cells
Concentration: 3 μM
Incubation Time: 48 hours
Result: Markedly reduced the tumor cell number.

Cell Proliferation Assay[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Decreased the rate of cellular proliferation in a dose-dependent manner, with an IC50 of 22.9 µM.

Cell Cycle Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Caused the G1 arrest.

Apoptosis Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM
Incubation Time: 24 hours, 48 hours
Result: Increased the level of cleaved caspase-3.

体内研究
(In Vivo)

C8-ceramide (0.1 mg/kg; intranasal administration) induces more robust CD8+ and CD4+ T cell responses to viral infections in virus infected mice[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice, with lymphocytic choriomeningitis virus infected[3]
Dosage: 0.1 mg/kg
Administration: Intranasal administration
Result: Increased the CD8+ T cell response to influenza in the lungs.

分子量

425.69

Formula

C26H51NO3
CAS 号

74713-59-0
中文名称

N-辛酰基-D-神经鞘氨醇
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
参考文献

  • [1]. Rebeca López-Marure , et al. Ceramide promotes the death of human cervical tumor cells in the absence of biochemical and morphological markers of apoptosis. Biochem Biophys Res Commun. 2002 May 10;293(3):1028-36.

    [2]. Yuli C. Chang, et al. Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells. Int J Mol Sci. 2018 Oct; 19(10): 3010.

    [3]. Curtis J. Pritzl, et al. A ceramide analogue stimulates dendritic cells to promote T cell responses upon virus infections. J Immunol. 2015 May 1; 194(9): 4339-4349.

    [4]. H W Huang, et al. Ceramides modulate protein kinase C activity and perturb the structure of Phosphatidylcholine/Phosphatidylserine bilayers. Biophys J. 1999 Sep; 77(3): 1489-1497.

    [5]. Lan Weiss, et al. Ceramide contributes to pathogenesis and may be targeted for therapy in VCP inclusion body myopathy. Hum Mol Genet. 2021 Jan 7;ddaa248.

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