Siomycin A(Synonyms: 盐霉素 A)

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Siomycin A (Synonyms: 盐霉素 A)

Siomycin A 是一种硫肽抗生素，也是一种 Forkhead box M1(FOXM1) 选择性抑制剂，而且不影响 Forkhead box 家族其他成员。Siomycin A 具有抗肿瘤作用并促进细胞凋亡。

Siomycin A(Synonyms: 盐霉素 A)

Siomycin A Chemical Structure

CAS No. : 12656-09-6

规格 是否有货
500 μg 询价

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生物活性

Siomycin A is a thiopeptide antibiotic and is a Forkhead box M1(FOXM1) selective inhibitor without affecting other members of the Forkhead box family. Siomycin A has anti-tumor and promotes apoptosis[1][2].

IC50 & Target

Forkhead box M1(FOXM1)[1]
体外研究
(In Vitro)

Siomycin A (0-10 µM; 24-72 hours; K562, MCF7 and MiaPaCa-2 cells) treatment markedly reduces cell viability in a dose-dependent and a time-dependent association in K562, MCF7 and MiaPaCa-2 cells. Among the three cell lines, the IC50 of the human leukemia K562 cells is the lowest at 6.25 µM at 24 h, while that for the human pancreatic cancer MiaPaCa-2 cells is 6.38 µM. However, the IC50 of the human pancreatic cancer MiaPaCa-2 cells at 48 and 72 h are the lowest of the three cell lines, which are 0.76 and 0.54 µM, respectively[1].
Siomycin A (0-10 µM; MiaPaCa-2 cells) treatment has potent proapoptotic effect in MiaPaCa-2 cells[1].
Siomycin A (0-10 µM; 24 hours; MiaPaCa-2 cells) treatment significantly reduces the expression levels of MMP-2, MMP-9 and α-tubulin protein in the MiaPaCa-2 cells[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: K562, MCF7 and MiaPaCa-2 cells
Concentration: 0 µM, 0.625 µM, 1.25 µM, 2.5 µM, 5 µM or 10 µM
Incubation Time: 24, 48 and 72 hours
Result: Cell viability was markedly reduced in a dose-dependent and a time-dependent association.

Apoptosis Analysis[1]

Cell Line: MiaPaCa-2 cells
Concentration: 0 µM, 0.625 µM, 1.25 µM, 2.5 µM, 5 µM or 10 µM
Incubation Time:
Result: Promoted apoptosis of MiaPaCa-2 cells.

Western Blot Analysis[1]

Cell Line: MiaPaCa-2 cells
Concentration: 0 µM, 0.625 µM, 1.25 µM, 2.5 µM, 5 µM or 10 µM
Incubation Time: 24 hours
Result: The expression levels of MMP-2 and MMP-9 protein in the MiaPaCa-2 cells were significantly reduced in the 2.5, 5 and 10 µM groups.

体内研究
(In Vivo)

Siomycin A (1 µM; subcutaneous injection; for 4 weeks; male Balb/c nude mice) pretreatment decreases tumour growth in an in vivo mouse model[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Four- to five-week old male Balb/c nude mice with IOMM-LEE cells[2]
Dosage: 1 µM-pretreated IOMM-Lee cells
Administration: Subcutaneous injection; for 4 weeks
Result: Decreased tumour growth in an in vivo mouse model.

分子量

1648.84

Formula

C71H81N19O18S5
CAS 号

12656-09-6
中文名称

盐霉素 A
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Wang B, et al. Effects and mechanism of siomycin A on the growth and apoptosis of MiaPaCa-2 cancer cells. Oncol Lett. 2019 Sep;18(3):2869-2876.

    [2]. Kim H, et al. Forkhead box M1 (FOXM1) transcription factor is a key oncogenic driver of aggressive human meningioma progression. Neuropathol Appl Neurobiol. 2019 Jun 9.

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