Fluensulfone is a new nematicide for chemical control of plant parasitic nematodes.

Lower concentrations of Fluensulfone delay development: 100 μM Fluensulfone causes a slight delay as at 66 h fewer worms have reached the adult stage whilst at 300 μM no worms reach the adult stage at 66 h and some fail to reach L4. Adult hermaphrodites lay fewer eggs in the presence of 1 mM Fluensulfone. Fluensulfone is also found to reduce the viability of eggs. After 3 h incubation with 100 μM to 1 mM Fluensulfone the thrashing rate is significantly inhibited, with maximal inhibition occurring with 1 mM. After 1 h both 300 μM and 1 mM Fluensulfone cause a significant and reversible inhibition of pharyngeal pumping relative to the vehicle control. Fluensulfone (500 μM) inhibits the frequency of body bends in one day old adult hermaphrodites off food after 2 h exposure^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

In an in vivo investigation, female mice are treated with Fluensulfone (or isoniazid as a positive control) for 3 and 7 days. Quantification of the cell proliferation by manual counting of BrdU-positive and BrdU-negative cells in the bronchiolar epithelium reveals an approximately fourfold increase of cell proliferation upon treatment with Fluensulfone and the positive control isoniazid compare with control. Increased cell proliferation is observed at 3 days but have reverted to the control level at day 7 ^[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

291.70

C₇H₅ClF₃NO₂S₂

318290-98-1

Room temperature in continental US; may vary elsewhere.

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

In Vitro: 

DMSO : 100 mg/mL (342.82 mM; Need ultrasonic)

H₂O : 0.73 mg/mL (2.50 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 2.5 mg/mL (8.57 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (8.57 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.5 mg/mL (8.57 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (8.57 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.5 mg/mL (8.57 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (8.57 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Kearn J, et al. Fluensulfone is a nematicide with a mode of action distinct from anticholinesterases and macrocyclic lactones. Pestic Biochem Physiol. 2014 Feb;109:44-57.

  [2]. Strupp C, et al. Relationship of metabolism and cell proliferation to the mode of action of fluensulfone-induced mouse lung tumors: analysis of their human relevance using the IPCS framework. Toxicol Sci. 2012 Jul;128(1):284-94.

For these assays worms of different developmental stages are incubated in liquid with and without Fluensulfone for up to 24 h, and paralysis is scored. 400 μL of M9 phosphate buffer with either Fluensulfone (100 μM, 200 μM or 1 mM) or vehicle (0.5% acetone) is put into each well of a 24 well plate (5 replicates for each Fluensulfone concentration). 5 μL suspension of age synchronised C. elegans (L1, L2/3, L4 or one day old adult) is added to each well. Each well contains approximately 50 to100 worms. The number of worms not moving at 1, 2, 3, 4, 5, 6 and 24 h is determined. The experiment is conducted on two separate occasions with five replicates^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Groups of 10 female specific pathogen-free CD-1 mice each are treated with untreated diet, diet containing 1200 mg/kg Fluensulfone (high dose in carcinogenicity study), or 1305 mg/kg of isoniazid as a positive control substance for 3 or 7 days, respectively. Two and 14 h before sacrifice, the animals are injected ip with 100 μL of a 10 mg/mL aqueous bromodeoxyuridine (BrdU)-solution. Sacrifice by exsanguination under deep irreversible pentobarbital narcosis is performed early in the morning to assure that the animals are exposed to the test item until shortly before sacrifice^[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Kearn J, et al. Fluensulfone is a nematicide with a mode of action distinct from anticholinesterases and macrocyclic lactones. Pestic Biochem Physiol. 2014 Feb;109:44-57.

  [2]. Strupp C, et al. Relationship of metabolism and cell proliferation to the mode of action of fluensulfone-induced mouse lung tumors: analysis of their human relevance using the IPCS framework. Toxicol Sci. 2012 Jul;128(1):284-94.