Ned 19 是一种选择性膜透性非竞争性 NAADP 拮抗剂，抑制 NAADP 介导的 Ca2+ 信号传导，IC50 为 65 nM。 Ned 19 强烈抑制小鼠的肿瘤生长和血管形成以及肺转移。
Ned 19 Chemical Structure
CAS No. : 874374-25-1
规格
是否有货
5 mg
询价
10 mg
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50 mg
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100 mg
询价
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生物活性
Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2].
IC50 & Target
Ca2+
体外研究 (In Vitro)
Ned 19 (25-100 μM; 24-72 hours) reduces cell proliferation[2]. Ned 19 (25-100 μM; 24-72 hours) reduces markedly the cell number[2]. Ned 19 (25-100 μM; 24-72 hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis[2]. Ned 19 (25-100 μM; 24-72 hours) induces cell apoptosis a time-dependent manner[2]. Ned 19 (25-100 μM; 24-72 hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior[2].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay[2]
Cell Line:
B16 cells
Concentration:
25, 50, 100 μM
Incubation Time:
24, 48, 72 hours
Result:
Reduced cell proliferation.
Cell Viability Assay[2]
Cell Line:
B16 cells
Concentration:
25, 50, 100 μM
Incubation Time:
24, 48, 72 hours
Result:
Reduced markedly the cell number.
Cell Cycle Analysis[2]
Cell Line:
B16 cells
Concentration:
25, 50, 100 μM
Incubation Time:
24, 48, 72 hours
Result:
Reduced the S phase percentage and increased of the G0/G1 phase percentage.
Apoptosis Analysis[2]
Cell Line:
B16 cells
Concentration:
25, 50, 100 μM
Incubation Time:
24, 48, 72 hours
Result:
Induced cell apoptosis a time-dependent manner.
Western Blot Analysis[2]
Cell Line:
B16 cells
Concentration:
25, 50, 100 μM
Incubation Time:
24, 48, 72 hours
Result:
Reduced expression of N-cadherin and increased expression of E-cadherin.
体内研究 (In Vivo)
Ned 19 (i.p.; 5 mg/Kg/every second day; 4 week) impaires severely tumor growth[2].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Adult male C57BL/6 mice[2]
Dosage:
5 mg/Kg
Administration:
I.p.; every second day; 4 week
Result:
Impaired severely tumor growth.
分子量
514.59
Formula
C30H31FN4O3
CAS 号
874374-25-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Rosen D, et al. Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonistNed-19 indicate two binding sites on the NAADP receptor. J Biol Chem. 2009 Dec 11;284(50):34930-4.
[2]. Annarita Favia, et al. NAADP-Dependent Ca2+ Signaling Controls Melanoma Progression, Metastatic Dissemination and Neoangiogenesis. Sci Rep. 2016; 6: 18925.
