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Aurora A inhibitor 2
Aurora A inhibitor 2 (Compound 16h) 是一个有效的 Aurora A kinase 抑制剂，其 IC50 值为21.94 nM。Aurora A inhibitor 2 诱导 MDA-MB-231 细胞 caspase 依赖性凋亡 (apoptosis)。
Aurora A inhibitor 2 Chemical Structure
CAS No. : 2412144-74-0
| 规格 |
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是否有货 |
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| 100 mg |
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询价 |
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| 250 mg |
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询价 |
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| 500 mg |
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询价 |
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* Please select Quantity before adding items.
| 生物活性 |
Aurora A inhibitor 2 (Compound 16h) is a potent Aurora A kinase inhibitor with an IC50 of 21.94 nM. Aurora A inhibitor 2 induces caspase-dependent apoptosis in MDA-MB-231 cells[1].
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| IC50 & Target |
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Aurora A
21.94 nM (IC50)
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Aurora B
273.18 nM (IC50)
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体外研究
(In Vitro) |
Aurora A inhibitor 2 (Compound 16h) (0-20 µM, 48 h) shows potent antiproliferative activity against various human cancer cells, and inhibits colony formation[1].
Aurora A inhibitor 2 (0-4 µM, 24 h) inhibits the expression of phosphorylation of Aurora A and Histone H3 in a dose-dependent manner, and induces G2/M cell cycle arrest [1].
Aurora A inhibitor 2 (0-4 µM, 48 h) induces obvious apoptosis in MDA-MB-231 cells in a concentration-dependent manner[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Cytotoxicity Assay[1]
| Cell Line: |
Breast cancer MDA-MB231, prostate cancer PC3, and neuroblastoma SH-SY5Y cells |
| Concentration: |
0-20 µM |
| Incubation Time: |
48 h |
| Result: |
Displayed potent antiproliferative activity with IC50 values of 0.38 ± 0.08, 1.09 ± 0.24, and 0.77 ± 0.12 µM against MDA-MB-231, PC3, and SH-SY5Y cells. |
Western Blot Analysis[1]
| Cell Line: |
MDAMB-231 |
| Concentration: |
1, 2, and 4 µM |
| Incubation Time: |
24 h |
| Result: |
A dose-dependent and significant reduction in the phosphorylation of Aurora A and Histone H3 was observed. Significantly increased the levels of cleaved caspase 3/9 and cleaved-PARP. |
Cell Cycle Analysis[1]
| Cell Line: |
MDAMB-231 |
| Concentration: |
1, 2, and 4 µM |
| Incubation Time: |
24 h |
| Result: |
Dose-dependently increased the and population of cells in the G2/M phase. |
Apoptosis Analysis[1]
| Cell Line: |
MDAMB-231 |
| Concentration: |
1, 2, and 4 µM |
| Incubation Time: |
48 h |
| Result: |
Induced obvious apoptosis in a concentration-dependent manner. |
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| CAS 号 |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 参考文献 |
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[1]. Chengcheng Fan, et al. Design, synthesis, biological evaluation of 6-(2-amino-1H-benzo[d]imidazole-6-yl)quinazolin-4(3H)-one derivatives as novel anticancer agents with Aurora kinase inhibition. Eur J Med Chem. 2020 Mar 15;190:112108.
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