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Erucin (Synonyms: 芥酸精； 甘油三芥酸酯)
Erucin (ERU) 是一种异硫氰酸盐 (isothiocyanate)，在芝麻菜 (arugula) 中尤其丰富。Erucin 具有抗癌 (anticancer)、神经保护和抗炎活性。
Erucin Chemical Structure
CAS No. : 4430-36-8
| 规格 |
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是否有货 |
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| 100 mg |
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询价 |
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| 250 mg |
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询价 |
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| 500 mg |
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询价 |
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| 生物活性 |
Erucin (ERU) is an isothiocyanate particularly abundant in arugula. Erucin shows anticancer, neuroprotective, and anti-inflammatory activities[1][2][3][4].
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体外研究
(In Vitro) |
Erucin (ERU) (0-100 μM) releases H2S and inhibits cell viability in AsPC‐1 cells in a concentration-dependent manner[1].
Erucin inhibits cell migration and altered the AsPC‐1 cell cycle, reducing G0/G1 phase and increasing G2/M and S phases[1].
Erucin (30 μM, 72 h) induces AsPC‐1 cell apoptosis and inhibits cell migration[1].
Erucin reduces levels of phosphorylated ERK1/2 in AsPC‐1 cells[1].
Erucin (0-200 μM, 24 h) shows antiproliferative activity with an IC50 of 97.7 µM in A549 cells[2].
Erucin (0-50 μM, 24 h) induces the cleavage of PARP-1 at 50 µM, and increases p53 and p21 protein expression in A549 cells[2].
Erucin decreases LPS-induced production of NO, prostaglandin E2 (PGE2), TNF-α, IL-6 and IL-1β in RAW 264.7 cells[3].
Erucin decreases LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in RAW 264.7 cells[3].
Erucin inhibits LPS-induced activation of NFκB Signaling in RAW 264.7 cells[3].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
| Cell Line: |
AsPC‐1 |
| Concentration: |
10, 30, and 100 μM |
| Incubation Time: |
72 h |
| Result: |
Showed a significant and concentration‐dependent reduction of cell viability. |
Cell Cycle Analysis[1]
| Cell Line: |
AsPC‐1 |
| Concentration: |
30 μM |
| Incubation Time: |
72 h |
| Result: |
Showed a particular increase of cells number in the G2/M phase (36.6% ± 3.5 vs. vehicle‐treated cells in the G2/M phase: 24.0% ± 1.3) and in the S‐phase (18.1% ± 1.5 vs. vehicle‐treated cells in the S phase: 11.0% ± 0.7) and a consequent significant reduction of cells in the G0/G1 phase (35.1% ± 5.0 vs. vehicle‐treated cells in the G0/G1 phase: 59.5% ± 1.8. |
Apoptosis Analysis[1]
| Cell Line: |
AsPC‐1 |
| Concentration: |
30 μM |
| Incubation Time: |
72 h |
| Result: |
Significantly increased the number of total apoptotic cells (apoptotic dead cells and apoptotic live cells; vehicle: 17.7% ± 2.5 vs. Erucin: 28.7% ± 4.2). |
Cell Proliferation Assay[2]
| Cell Line: |
A549 |
| Concentration: |
0-200 µM |
| Incubation Time: |
24 h |
| Result: |
Showed antiproliferative effect with an IC50 of 97.7 µM. |
Western Blot Analysis[2]
| Cell Line: |
A549 |
| Concentration: |
0-50 µM |
| Incubation Time: |
24 h |
| Result: |
Induced the cleavage of PARP-1 at 50 µM. Increased p53 and p21 protein expression. |
Western Blot Analysis[3]
| Cell Line: |
RAW 264.7 |
| Concentration: |
0, 2.5, and 5 µM |
| Incubation Time: |
30 min |
| Result: |
Decreased the expression of iNOS and COX-2 induced by LPS. Suppressed the LPS-induced reduction in IκB-α. Suppressed NFκB DNA binding and transcriptional activity. |
RT-PCR[3]
| Cell Line: |
RAW 264.7 |
| Concentration: |
0, 2.5, and 5 µM |
| Incubation Time: |
24 h |
| Result: |
Decreased LPS-induced TNF-α, IL-6 and IL-1β production. |
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体内研究
(In Vivo) |
Erucin (ERU) (0-300 nM) significantly inhibits TPA-induced edema formation[3]. Erucin (30 μmol/kg; i.p.; twice a week for 4 week) shows neuroprotective effects[4].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: |
Female ICR mice (4 weeks of age), TPA (12-O-tetradecanoylphorbol-13-acetate)-induced mouse ear edema model[3] |
| Dosage: |
0, 100, and 300 nM |
| Administration: |
Topically applied to the mouse ear 30 min prior to the topical application of TPA |
| Result: |
Significantly inhibited TPA-induced edema formation. |
| Animal Model: |
Male C57Bl/6 mice (9 weeks old, 25–30 g body weight)[4] |
| Dosage: |
30 μmol/kg |
| Administration: |
Intraperitoneal administration, twice a week, 4 weeks (Induce brain lesion by intrastriatal injection of 6-OHDA). |
| Result: |
Induced a partial recovery in the rotational behavior test. Upregulated the expression of TH. Counteract neuronal death and DNA fragmentation in 6-OHDA lesioned mice. increase total GSH and Nrf2 levels in 6-OHDA lesioned mice. |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 参考文献 |
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[1]. Valentina Citi, et al. Anticancer properties of erucin, an H2 S-releasing isothiocyanate, on human pancreatic adenocarcinoma cells (AsPC-1). Phytother Res. 2019 Mar;33(3):845-855.
[2]. A. Melchini, et al. Erucin, a new promising cancer chemopreventive agent from rocket salads, shows anti-proliferative activity on human lung carcinoma A549 cells. Food Chem Toxicol. 2009 Jul;47(7):1430-6.
[3]. Han Jin Cho, et al. Erucin exerts anti-inflammatory properties in murine macrophages and mouse skin: possible mediation through the inhibition of NFκB signaling. Int J Mol Sci. 2013 Oct 15;14(10):20564-77.
[4]. Fabiana Morroni, et al. Comparison of Adaptive Neuroprotective Mechanisms of Sulforaphane and its Interconversion Product Erucin in in Vitro and in Vivo Models of Parkinson’s Disease. J Agric Food Chem. 2018 Jan 31;66(4):856-865.
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