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Imipramine (Synonyms: 米帕明)
Imipramine 是一种具有口服活性的叔胺类三环抗抑郁药。Imipramine 是一种具有抗肿瘤活性的 Fascin1 抑制剂。Imipramine 刺激 U-87MG 胶质瘤细胞自噬（autophagy），诱导 HL-60 细胞凋亡（apoptosis）。Imipramine 具有神经保护和免疫调节作用。
Imipramine Chemical Structure
CAS No. : 50-49-7
| 规格 |
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是否有货 |
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| 100 mg |
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询价 |
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| 250 mg |
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询价 |
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| 500 mg |
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询价 |
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* Please select Quantity before adding items.
Imipramine 的其他形式现货产品:
Imipramine hydrochloride
| 生物活性 |
Imipramine is an orally active tertiary amine tricyclic antidepressant. Imipramine is a Fascin1 inhibitor with antitumor activities. Imipramine stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine shows neuroprotective and immunomodulatory effects[1][2][3][4].
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IC50 & Target |
Fascin1, Autophagy, Apoptosis[1][2][3]
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体外研究
(In Vitro) |
Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability[1].
Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h)[1].
Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells[2].
Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy[2].
Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis[3].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
| Cell Line: |
DLD-1, HCT-116, and SW-480 |
| Concentration: |
0.5-300 μM |
| Incubation Time: |
3 days |
| Result: |
Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480. |
Cell Migration Assay [1]
| Cell Line: |
DLD-1, HCT-116, and SW-480 |
| Concentration: |
20 μM |
| Incubation Time: |
7 h |
| Result: |
Produced a remarkable inhibition of migration in all assayed cell lines. |
Cell Invasion Assay[1]
| Cell Line: |
HCT-116 |
| Concentration: |
20 μM |
| Incubation Time: |
48 h |
| Result: |
Inhibited cell invasion through Matrigel. |
Western Blot Analysis[2]
| Cell Line: |
U-87MG |
| Concentration: |
50 μM |
| Incubation Time: |
0, 15, 30, 60, 120 and 240 min |
| Result: |
Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR. |
Cell Autophagy Assay[2]
| Cell Line: |
U-87MG |
| Concentration: |
60 μM |
| Incubation Time: |
24 h |
| Result: |
Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells. |
Apoptosis Analysis[3]
| Cell Line: |
HL-60 |
| Concentration: |
80 μM |
| Incubation Time: |
24 h |
| Result: |
Induced cell apoptosis. |
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体内研究
(In Vivo) |
Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice[4].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: |
Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)[4] |
| Dosage: |
20 mg/kg or 15 mg/kg |
| Administration: |
Intraperitoneal injection or oral administration, daily for 24 days |
| Result: |
Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia. |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 参考文献 |
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[1]. Alburquerque-González B, et al. New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells. Exp Mol Med. 2020 Feb;52(2):281-292.
[2]. Jeon SH, et al. The tricyclic antidepressant imipramine induces autophagic cell death in U-87MG glioma cells. Biochem Biophys Res Commun. 2011 Sep 23;413(2):311-7.
[3]. Xia Z, et al. The antidepressants imipramine, clomipramine, and citalopram induce apoptosis in human acute myeloid leukemia HL-60 cells via caspase-3 activation. J Biochem Mol Toxicol. 1999;13(6):338-47.
[4]. Ramirez K, et al. Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Brain Behav Immun. 2015 May;46:212-20.
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