Psoralen (Ficusin) is a coumarin isolated from the seeds of Fructus Psoraleae. Psoralen exhibits a wide range of biological properties, including anti-cancer, antioxidant, antidepressant, anticancer, antibacterial, and antiviral, et al^[1].

Psoralen (10-500 μM; 24-48 hours) inhibits cell viability in a concentration- and time-dependent manner in L02 and HepG2 cells. In L02 cells, Psoralen at 400 μM does not significantly change extracellular LDH levels, and 400 μM or 450 μM psoralen inhibits 50–60% of cell viability^[1].
Psoralen (150-450 μM; 24 hours) induces significant S-phase arrest in L02 cells in time- and dose-dependent manners, but it does not exhibits significant change in the cycle distribution of HepG2 cells^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Psoralen (oral gavage; 17.5 mg/kg; 6 weeks) reduces the number of metastatic lesions and the rate of bone metastasis by 20% compared to vehicle-treated mice. It also reduces tumor infiltration and decreases the percentage of tumor cells in metastatic lesions by ~40% compared to vehicle in mice^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

186.16

C₁₁H₆O₃

66-97-7

补骨脂素；补骨脂内酯；补骨酯素

Room temperature in continental US; may vary elsewhere.

DMSO : 100 mg/mL (537.17 mM; Need ultrasonic)

H₂O : 1 mg/mL (5.37 mM; ultrasonic and warming and heat to 80°C)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (13.43 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: 2.5 mg/mL (13.43 mM); Suspended solution; Need ultrasonic

  此方案可获得 2.5 mg/mL (13.43 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (13.43 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Li Yin, et al. A novel psoralen derivative-MPFC enhances melanogenesis via activation of p38 MAPK and PKA signaling pathways in B16 cells. Int J Mol Med. 2018 Jun;41(6):3727-3735.

  [2]. Wu C, et al. Psoralen inhibits bone metastasis of breast cancer in mice. Fitoterapia. 2013 Dec;91:205-10.