Destruxin B 是从昆虫病原真菌中分离的绿僵菌 (Metarhizium anisopliae)，是一种具有杀虫和抗癌活性的环肽。 Destruxin B 通过 Bcl-2 家族依赖的线粒体途径在人类非小细胞肺癌细胞中诱导细胞凋亡。Destruxin B 显著激活 caspase-3，并在体外和体内通过 caspase 介导的细胞凋亡减少肿瘤细胞增殖。
Destruxin B Chemical Structure
CAS No. : 2503-26-6
规格
价格
是否有货
数量
1 mg
￥10500
In-stock
5 mg
￥27500
询价
10 mg
询价
50 mg
询价
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生物活性
Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells[1]. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo[2].
IC50 & Target
Bcl-2
Caspase-3
体外研究 (In Vitro)
Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC50s of 4.1 μM and 4.9 μM, respectively[1]. Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells[1]. Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners[2]. Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner [1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay[1]
Cell Line:
H1299 cells
Concentration:
1, 5, 10, 20, 30 μM
Incubation Time:
48 hours
Result:
Inhibited H1299 cells proliferation of H1299 cells with an IC50 of 4.1 μM.
The IC50s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively.
Western Blot Analysis[1]
Cell Line:
A549 cells
Concentration:
10 or 20 μM
Incubation Time:
12 and 24 hours
Result:
Regulates Mcl-1 and PUMA.
体内研究 (In Vivo)
Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners[2].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival[2]
Dosage:
Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg)
Administration:
Injection; daily; for 6 weeks
Result:
Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively.
分子量
621.81
Formula
C32H55N5O7
CAS 号
2503-26-6
中文名称
黑僵菌素 B
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
参考文献
[1]. Wu CC, et al. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human NonsmallCell Lung Cancer Cells. Evid Based Complement Alternat Med. 2013;2013:548929.
[2]. Lee YP, et al. In vitro and in vivo anticancer effects of destruxin B on human colorectal cancer. Anticancer Res. 2012 Jul;32(7):2735-45.
