Ferulic acid methyl ester(Synonyms: 阿魏酸甲酯; Methyl ferulate)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Ferulic acid methyl ester (Synonyms: 阿魏酸甲酯; Methyl ferulate) 纯度: 99.93%

Ferulic acid methyl ester (Methyl ferulate) 是从 Stemona tuberosa 中分到的阿魏酸衍生物,具有抗氧化、抗炎作用。Ferulic acid methyl ester 能够透过细胞膜和血脑屏障,清除自由基,可用于神经退行性疾病的研究。在原发性骨髓源性巨噬细胞中,Ferulic acid methyl ester 抑制 COX-2 的表达,降低 p-p38 和 p-JNK 的水平。

Ferulic acid methyl ester(Synonyms: 阿魏酸甲酯; Methyl ferulate)

Ferulic acid methyl ester Chemical Structure

CAS No. : 2309-07-1

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Ferulic acid methyl ester 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Kinase Inhibitor Library
  • MAPK Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • CNS-Penetrant Compound Library
  • Autophagy Compound Library
  • Reprogramming Compound Library
  • Oxygen Sensing Compound Library
  • Phenols Library
  • Pyroptosis Compound Library
  • Anti-Lung Cancer Compound Library
  • Neurodegenerative Disease-related Compound Library
  • Angiogenesis Related Compound Library
  • Food-Sourced Compound Library

生物活性

Ferulic acid methyl ester (Methyl ferulate) is a derivative of ferulic acid, isolated from Stemona tuberosa, with anti-inflammatory and antioxidant properties[1][2]. Ferulic acid methyl ester is a cell membrane and brain permeable compound, shows free radical scavenging ability, used in the research of neurodegenerative disorders[1]. Ferulic acid methyl ester inhibits COX-2 expression, blocks p-p38 and p-JNK in primary bone marrow derived-macrophages[2].

IC50 & Target[2]

p38

 

体外研究
(In Vitro)

Ferulic acid methyl ester (25 µg/mL) has no cytotoxic effects on BMDMs after treatment for 6 h, 18 h, 48 h[2].
Ferulic acid methyl ester (Methyl ferulate; 5, 10, 25 µg/mL) suppresses TNFα, IL6, IFNγ but not IL10, inhibits NO generation at 10 and 25 µg/mL, in primary bone marrow derived-macrophages (BMDMs)[2].
Ferulic acid methyl ester (25 µg/mL) inhibits COX-2 expression, blocks p-p38 and p-JNK[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Primary bone marrow derived-macrophages (BMDMs)
Concentration: 25 µg/mL
Incubation Time: 6 h, 18 h, 48 h
Result: Showed no cytotoxic effects on BMDMs.

Western Blot Analysis[2]

Cell Line: RAW 246.7 cells
Concentration: 10 µg/mL and 25 µg/mL
Incubation Time: For 1 h before stimulation with LPS
Result: Significantly suppressed COX-2 expression at 25 µg/mL.

分子量

208.21

Formula

C11H12O4

CAS 号

2309-07-1

中文名称

阿魏酸甲酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL (480.28 mM)

H2O : 2 mg/mL (9.61 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.8028 mL 24.0142 mL 48.0284 mL
5 mM 0.9606 mL 4.8028 mL 9.6057 mL
10 mM 0.4803 mL 2.4014 mL 4.8028 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (12.01 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (12.01 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (12.01 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Sultana R. Ferulic acid ethyl ester as a potential therapy in neurodegenerative disorders. Biochim Biophys Acta. 2012 May;1822(5):748-52.

    [2]. Phuong NT, et al. Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour. Asian Pac J Trop Med. 2014 Sep;7S1:S327-31.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务