Lupiwighteone is an isoflavone present widely in wild-growing plants, with antioxidant, antimicrobial and anticancer effects. Lupiwighteone induces caspase-dependent and -independent apoptosis on human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway[1][2].
IC50 & Target
apoptosis[2]
体外研究 (In Vitro)
Lupiwighteone (2-100 μM; 72 hours) shows cytotoxicity towards various cell lines, especially on DU-145 cells and SGC-7901 cells with IC50s of 23.7 μM and 21 μM, respectively[2]. Lupiwighteone (20-60 μM; 48 hours) induces (cell cycle arrest in DU-145 cells[2]. Lupiwighteone (20-60 μM; 48 hours) induces cells apoptosis[2]. Lupiwighteone (20-60 μM; 48 hours) decreases the cell cycle-related protein expressions in a dose-dependent manner in DU-145 cells[2]. Lupiwighteone (20-60 μM; 48 hours) induces a dose-dependent increase in ROS production[2]. Lupiwighteone up-regulates of cytochrome c and caspase-3, and subsequent cleavage of PARP-1 and down-regulates of the p-Akt/Akt ratio and VEGF expression, suggests the activation of mitochondria-based intrinsic apoptosis in DU-145 cells[2].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[2]
Cell Line:
DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero
Concentration:
2 μM, 5 μM, 25 μM, 50 μM, 100 μM
Incubation Time:
72 hours
Result:
Had inhibition on the survival of many cancer cell lines and HUVEC.
Cell Cycle Analysis[2]
Cell Line:
DU-145 cells
Concentration:
20 μM, 40 μM, 60 μM
Incubation Time:
48 hours
Result:
Induced cell cycle arrest.
Apoptosis Analysis[2]
Cell Line:
DU-145 cells
Concentration:
20 μM, 40 μM, 60 μM
Incubation Time:
48 hours
Result:
Induced apoptosis.
Western Blot Analysis[2]
Cell Line:
DU-145 cells
Concentration:
20 μM, 40 μM, 60 μM
Incubation Time:
48 hours
Result:
Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner.
分子量
338.35
Formula
C20H18O5
CAS 号
104691-86-3
中文名称
黄羽扇豆魏特酮
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Won YS, et al. Lupiwighteone induces caspase-dependent and -independent apoptosis on human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway. Food Chem Toxicol. 2020 Jan;135:110863.
[2]. Ren J, et al. Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells. Anticancer Drugs. 2015 Jul;26(6):599-611.
